Dr. Jyoti K. Jaiswal, MSC, PhD

Associate Professor
Departments of Genomics and Precision Medicine and Pediatrics
George Washington University School of Medicine and Health Sciences

Hangout Title: "How cells survive in the minutes following an injury"  
Hangout Schedule: Dec 6th: 10.30 am EST, 9.30 am CST, 7.30 am PST, 9 pm IST


Academic Appointment(s)
Associate Professor of Integrative Systems Biology, George Washington University School of Medicine and Health Sciences
Associate Professor of Pediatrics, George Washington University School of Medicine and Health Sciences

Research Interests
  • Cell biology
  • Lysosome
  • Muscular dystrophy
  • Injury
  • Inflammation
  • Microscopy
  • Nanotechnology

Publications (Google Scholar citations till Dec 2017: >6200)

Refereed publications in Journals

1) Novak JS, Hogarth MW, Boehler JF, Nearing M, Vila MC, Heredia R, Fiorillo AA, Zhang A,
Hathout Y, Hoffman EP, Jaiswal JK, Nagaraju K, Cirak S, Partridge TA. Myoblasts and
macrophages are required for therapeutic morpholino antisense oligonucleotide delivery to
dystrophic muscle. Nat Commun. 2017 Oct 16;8(1):941. doi: 10.1038/s41467-017-00924-7.
PubMed PMID: 29038471; PubMed Central PMCID: PMC5643396.

2) Horn A, Van der Meulen JH, Defour A, Hogarth M, Sreetama SC, Reed A, Scheffer L,
Chandel NS, Jaiswal JK. Mitochondrial redox signaling enables repair of injured skeletal
muscle cells. Sci Signal. 2017 Sep 5;10(495). pii: eaaj1978. doi: 10.1126/scisignal.aaj1978.
PubMed PMID: 28874604.

3) Defour A, Medikayala S, Van der Meulen JH, Hogarth MW, Holdreith N, Malatras A, Duddy
W, Boehler J, Nagaraju K, Jaiswal JK. Annexin A2 links poor myofiber repair with inflammation
and adipogenic replacement of the injured muscle. Hum Mol Genet. 2017 Jun 1;26(11):1979-
1991. doi: 10.1093/hmg/ddx065. PubMed PMID: 28334824.

4) Li J, Das JR, Tang P, Han Z, Jaiswal JK, Ray PE. Transmembrane TNF-α Facilitates HIV-
1 Infection of Podocytes Cultured from Children with HIV-Associated Nephropathy. J Am Soc
Nephrol. 2017 Mar;28(3):862-875. doi: 10.1681/ASN.2016050564. Epub 2016 Nov 3. PubMed
PMID: 27811066; PubMed Central PMCID: PMC5328167.

5) Salka K, Bhuvanendran S, Wilson K, Bozidis P, Mehta M, Rainey K, Sesaki H, Patterson
GH, Jaiswal JK, Colberg-Poley AM. Superresolution Imaging Identifies That Conventional
Trafficking Pathways Are Not Essential for Endoplasmic Reticulum to Outer Mitochondrial
Membrane Protein Transport. Sci Rep. 2017 Feb 2;7(1):16. doi: 10.1038/s41598-017-00039-5.
PubMed PMID: 28154412; PubMed Central PMCID: PMC5428351.

6) Vila MC, Rayavarapu S, Hogarth MW, Van der Meulen JH, Horn A, Defour A, Takeda S,
Brown KJ, Hathout Y, Nagaraju K, Jaiswal JK. Mitochondria mediate cell membrane repair and
contribute to Duchenne muscular dystrophy. Cell Death Differ. 2017 Feb;24(2):330-342. doi:
10.1038/cdd.2016.127. Epub 2016 Nov 11. PubMed PMID: 27834955; PubMed Central PMCID:

7) Perovanovic J, Dell'Orso S, Gnochi VF, Jaiswal JK, Sartorelli V, Vigouroux C, Mamchaoui
K, Mouly V, Bonne G, Hoffman EP. Laminopathies disrupt epigenomic developmental programs
and cell fate. Sci Transl Med. 2016 Apr 20;8(335):335ra58. doi: 10.1126/scitranslmed.aad4991.
PubMed PMID: 27099177; PubMed Central PMCID: PMC4939618.

8) Sreetama SC, Takano T, Nedergaard M, Simon SM, Jaiswal JK. Injured astrocytes are
repaired by Synaptotagmin XI-regulated lysosome exocytosis. Cell Death Differ. 2016
Apr;23(4):596-607. doi: 10.1038/cdd.2015.124. Epub 2015 Oct 9. PubMed PMID: 26450452;
PubMed Central PMCID: PMC4986631.

9) Leikina E, Defour A, Melikov K, Van der Meulen JH, Nagaraju K, Bhuvanendran S, Gebert
C, Pfeifer K, Chernomordik LV, Jaiswal JK. Annexin A1 Deficiency does not Affect Myofiber
Repair but Delays Regeneration of Injured Muscles. Sci Rep. 2015 Dec 15;5:18246. doi:
10.1038/srep18246. PubMed PMID: 26667898; PubMed Central PMCID: PMC4678367. 

10) Colberg-Poley AM, Patterson GH, Salka K, Bhuvanendran S, Yang D, Jaiswal JK.
Superresolution imaging of viral protein trafficking. Med Microbiol Immunol. 2015
Jun;204(3):449-60. doi: 10.1007/s00430-015-0395-0. Epub 2015 Feb 28. Review. PubMed
PMID: 25724304; PubMed Central PMCID: PMC4441596.

11) Yadavilli S, Scafidi J, Becher OJ, Saratsis AM, Hiner RL, Kambhampati M, Mariarita S,
MacDonald TJ, Codispoti KE, Magge SN, Jaiswal JK, Packer RJ, Nazarian J. The emerging
role of NG2 in pediatric diffuse intrinsic pontine glioma. Oncotarget. 2015 May 20;6(14):12141-
55. PubMed PMID: 25987129; PubMed Central PMCID: PMC4494928.

12) Scheffer LL, Sreetama SC, Sharma N, Medikayala S, Brown KJ, Defour A, Jaiswal JK.
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. Nat
Commun. 2014 Dec 23;5:5646. doi: 10.1038/ncomms6646. PubMed PMID: 25534348; PubMed
Central PMCID: PMC4333728.

13) Defour A, Van der Meulen JH, Bhat R, Bigot A, Bashir R, Nagaraju K, Jaiswal JK.
Dysferlin regulates cell membrane repair by facilitating injury-triggered acid sphingomyelinase
secretion. Cell Death Dis. 2014 Jun 26;5:e1306. doi: 10.1038/cddis.2014.272. PubMed PMID:
24967968; PubMed Central PMCID: PMC4079937.

14) Jaiswal JK, Lauritzen SP, Scheffer L, Sakaguchi M, Bunkenborg J, Simon SM, Kallunki T,
Jäättelä M, Nylandsted J. S100A11 is required for efficient plasma membrane repair and
survival of invasive cancer cells. Nat Commun. 2014 May 8;5:3795. doi: 10.1038/ncomms4795.
PubMed PMID: 24806074; PubMed Central PMCID: PMC4026250.

15) Buvanendran S, Salka K, Rainey K, Sreetama SC, Williams E, Leeker M, Prasad V, Boyd
J, Patterson GH, Jaiswal JK, Colberg-Poley AM. Superresolution imaging of human
cytomegalovirus vMIA localization in sub-mitochondrial compartments. Viruses. 2014 Apr
9;6(4):1612-36. doi: 10.3390/v6041612. PubMed PMID: 24721787; PubMed Central PMCID:

16) Defour A, Sreetama SC, Jaiswal JK. Imaging cell membrane injury and subcellular
processes involved in repair. J Vis Exp. 2014 Mar 24;(85). doi: 10.3791/51106. PubMed PMID:
24686523; PubMed Central PMCID: PMC4089398.

17) Heier CR, Damsker JM, Yu Q, Dillingham BC, Huynh T, Van der Meulen JH, Sali A, Miller
BK, Phadke A, Scheffer L, Quinn J, Tatem K, Jordan S, Dadgar S, Rodriguez OC, Albanese C,
Calhoun M, Gordish-Dressman H, Jaiswal JK, Connor EM, McCall JM, Hoffman EP, Reeves
EK, Nagaraju K. VBP15, a novel anti-inflammatory and membrane-stabilizer, improves
muscular dystrophy without side effects. EMBO Mol Med. 2013 Oct;5(10):1569-85. doi:
10.1002/emmm.201302621. Epub 2013 Sep 9. PubMed PMID: 24014378; PubMed Central
PMCID: PMC3799580.

18) Rayavarapu S, Coley W, Cakir E, Jahnke V, Takeda S, Aoki Y, Grodish-Dressman H,
Jaiswal JK, Hoffman EP, Brown KJ, Hathout Y, Nagaraju K. Identification of disease specific
pathways using in vivo SILAC proteomics in dystrophin deficient mdx mouse. Mol Cell
Proteomics. 2013 May;12(5):1061-73. doi: 10.1074/mcp.M112.023127. Epub 2013 Jan 7.
PubMed PMID: 23297347; PubMed Central PMCID: PMC3650321. 

19) Fu X, Brown KJ, Yap CC, Winckler B, Jaiswal JK, Liu JS. Doublecortin (Dcx) family
proteins regulate filamentous actin structure in developing neurons. J Neurosci. 2013 Jan
9;33(2):709-21. doi: 10.1523/JNEUROSCI.4603-12.2013. PubMed PMID: 23303949; PubMed
Central PMCID: PMC3711551.

20) Macro L, Jaiswal JK, Simon SM. Dynamics of clathrin-mediated endocytosis and its
requirement for organelle biogenesis in Dictyostelium. J Cell Sci. 2012 Dec 1;125(Pt 23):5721-
32. doi: 10.1242/jcs.108837. Epub 2012 Sep 19. PubMed PMID: 22992464; PubMed Central
PMCID: PMC3575707.

21) Liu JS, Schubert CR, Fu X, Fourniol FJ, Jaiswal JK, Houdusse A, Stultz CM, Moores CA,
Walsh CA. Molecular basis for specific regulation of neuronal kinesin-3 motors by doublecortin
family proteins. Mol Cell. 2012 Sep 14;47(5):707-21. doi: 10.1016/j.molcel.2012.06.025. Epub
2012 Aug 1. PubMed PMID: 22857951; PubMed Central PMCID: PMC3549492.

22) Sharma N, Medikayala S, Defour A, Rayavarapu S, Brown KJ, Hathout Y, Jaiswal JK. Use
of quantitative membrane proteomics identifies a novel role of mitochondria in healing injured
muscles. J Biol Chem. 2012 Aug 31;287(36):30455-67. doi: 10.1074/jbc.M112.354415. Epub
2012 Jul 9. PubMed PMID: 22778268; PubMed Central PMCID: PMC3436295.

23) Johnson DS, Jaiswal JK, Simon S. Total internal reflection fluorescence (TIRF)
microscopy illuminator for improved imaging of cell surface events. Curr Protoc Cytom. 2012
Jul;Chapter 12:Unit 12.29. doi: 10.1002/0471142956.cy1229s61. PubMed PMID: 22752951.

24) Mahjneh I, Jaiswal J, Lamminen A, Somer M, Marlow G, Kiuru-Enari S, Bashir R. A new
distal myopathy with mutation in anoctamin 5. Neuromuscul Disord. 2010 Dec;20(12):791-5.
doi: 10.1016/j.nmd.2010.07.270. Epub 2010 Aug 7. PubMed PMID: 20692837; PubMed Central
PMCID: PMC4206776.

25) Jaiswal JK, Rivera VM, Simon SM. Exocytosis of post-Golgi vesicles is regulated by
components of the endocytic machinery. Cell. 2009 Jun 26;137(7):1308-19. doi:
10.1016/j.cell.2009.04.064. PubMed PMID: 19563761; PubMed Central PMCID: PMC3237386.

26) Jaiswal JK, Fix M, Takano T, Nedergaard M, Simon SM. Resolving vesicle fusion from
lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes. Proc Natl Acad Sci U S A.
2007 Aug 28;104(35):14151-6. Epub 2007 Aug 21. PubMed PMID: 17715060; PubMed Central
PMCID: PMC1955787.

27) Jirousková M, Jaiswal JK, Coller BS. Ligand density dramatically affects integrin alpha IIb
beta 3-mediated platelet signaling and spreading. Blood. 2007 Jun 15;109(12):5260-9. Epub
2007 Mar 1. PubMed PMID: 17332246; PubMed Central PMCID: PMC1890822.

28) Koeppel F, Jaiswal JK, Simon SM. Quantum dot-based sensor for improved detection of
apoptotic cells. Nanomedicine (Lond). 2007 Feb;2(1):71-8. PubMed PMID: 17716192.

29) Jaiswal JK, Marlow G, Summerill G, Mahjneh I, Mueller S, Hill M, Miyake K, Haase H,
Anderson LV, Richard I, Kiuru-Enari S, McNeil PL, Simon SM, Bashir R. Patients with a nondysferlin
Miyoshi myopathy have a novel membrane repair defect. Traffic. 2007 Jan;8(1):77-88.
Epub 2006 Nov 21. PubMed PMID: 17132147.

30) Jaiswal JK, Simon SM. Optical monitoring of single cells using quantum dots. Methods
Mol Biol. 2007;374:93-104. PubMed PMID: 17237532.

31) Jaiswal JK, Mujumdar N, Macwilliams HK, Nanjundiah V. Trishanku, a novel regulator of
cell-type stability and morphogenesis in Dictyostelium discoideum. Differentiation. 2006
Dec;74(9-10):596-607. PubMed PMID: 17177856.

32) Takano T, Kang J, Jaiswal JK, Simon SM, Lin JH, Yu Y, Li Y, Yang J, Dienel G, Zielke
HR, Nedergaard M. Receptor-mediated glutamate release from volume sensitive channels in
astrocytes. Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16466-71. Epub 2005 Oct 27.
PubMed PMID: 16254051; PubMed Central PMCID: PMC1283436.

33) Uyeda HT, Medintz IL, Jaiswal JK, Simon SM, Mattoussi H. Synthesis of compact
multidentate ligands to prepare stable hydrophilic quantum dot fluorophores. J Am Chem Soc.
2005 Mar 23;127(11):3870-8. PubMed PMID: 15771523.

34) Jaiswal JK, Goldman ER, Mattoussi H, Simon SM. Use of quantum dots for live cell
imaging. Nat Methods. 2004 Oct;1(1):73-8. PubMed PMID: 16138413.

35) Voura EB, Jaiswal JK, Mattoussi H, Simon SM. Tracking metastatic tumor cell
extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy.
Nat Med. 2004 Sep;10(9):993-8. Epub 2004 Aug 29. PubMed PMID: 15334072.

36) Jaiswal JK, Chakrabarti S, Andrews NW, Simon SM. Synaptotagmin VII restricts fusion
pore expansion during lysosomal exocytosis. PLoS Biol. 2004 Aug;2(8):E233. Epub 2004 Jun
29. PubMed PMID: 15226824; PubMed Central PMCID: PMC439782.

37) Fix M, Melia TJ, Jaiswal JK, Rappoport JZ, You D, Söllner TH, Rothman JE, Simon SM.
Imaging single membrane fusion events mediated by SNARE proteins. Proc Natl Acad Sci U S
A. 2004 May 11;101(19):7311-6. Epub 2004 May 3. PubMed PMID: 15123811; PubMed Central
PMCID: PMC409915.

38) Jaiswal JK, Nanjundiah V. Calcium regulates the expression of a Dictyostelium
discoideum asparaginyl tRNA synthetase gene. J Biosci. 2003 Dec;28(6):697-707. PubMed
PMID: 14660869.

39) Jaiswal JK, Simon SM. Total internal reflection fluorescence microscopy for highresolution
imaging of cell-surface events. Curr Protoc Cell Biol. 2003 Nov;Chapter 4:Unit 4.12.
doi: 10.1002/0471143030.cb0412s20. PubMed PMID: 18228434.

40) Jaiswal JK, Mattoussi H, Mauro JM, Simon SM. Long-term multiple color imaging of live
cells using quantum dot bioconjugates. Nat Biotechnol. 2003 Jan;21(1):47-51. Epub 2002 Dec
2. PubMed PMID: 12459736.

41) Jaiswal JK, Andrews NW, Simon SM. Membrane proximal lysosomes are the major
vesicles responsible for calcium-dependent exocytosis in nonsecretory cells. J Cell Biol. 2002
Nov 25;159(4):625-35. Epub 2002 Nov 18. PubMed PMID: 12438417; PubMed Central PMCID:

42) Jaiswal JK. Calcium - how and why? J Biosci. 2001 Sep;26(3):357-63. Review. PubMed
PMID: 11568481.

Invited Publications (Peer Reviewed)
1. Jaiswal JK, Simon SM. Imaging Live Cells Using Quantum Dots. Cold Spring Harb Protoc.
2015 Jul 1;2015(7):619-25. doi: 10.1101/pdb.top086322. PubMed PMID: 26134913.

2. Jaiswal JK, Nylandsted J. S100 and annexin proteins identify cell membrane damage as
the Achilles heel of metastatic cancer cells. Cell Cycle. 2015;14(4):502-9. doi:
10.1080/15384101.2014.995495. PubMed PMID: 25565331; PubMed Central PMCID:

3. Jaiswal JK, Simon SM. Belling the cat - tagging live cells with quantum dots. Clin Chem.
2013 Jun;59(6):995-6. doi: 10.1373/clinchem.2012.199166. Epub 2013 Jan 28. PubMed
PMID: 23358412.

4. Jaiswal JK, Simon SM. Imaging single events at the cell membrane. Nat Chem Biol. 2007
Feb;3(2):92-8. Review. PubMed PMID: 17235347.

5. Jaiswal, J.K, Voura, E.B., Mattoussi, H., Simon, S.M. Use of fluorescent quantum dots for
studying live cells and organisms. Proceedings of SPIE. (2005) 5705, 210-216.

6. Jaiswal JK, Simon SM. Potentials and pitfalls of fluorescent quantum dots for biological
imaging. Trends Cell Biol. 2004 Sep;14(9):497-504. Review. PubMed PMID: 15350978.

Chapters in Books (Peer Reviewed)
1. Johnson DS, Jaiswal JK, and Simon SM. (2012) Total Internal Reflection Fluorescence (TIRF)
Microscopy Illuminator for Improved Imaging of Cell Surface Events. Current Protocols in
Cytometry (2012); Unit 12.29. Simon Watkins (ed.) (John Wiley and Sons Inc., New Jersey).

2. Jaiswal JK and Simon SM. (2007) Optical monitoring of single cells using quantum dots.
Methods in Molecular Biology - Quantum Dots Application in Biology 374, chapter 8, 93-104.
Hotz C.Z. and Bruchez M. Jr. (eds.) (Humana Press, New Jersey).

3. Jaiswal, JK, Goldman ER, Mattoussi H, and Simon SM. (2005) Imaging with quantum dots.
Imaging in Neuroscience and Development. Chapter 66, 511-516. Yuste R and Konnerth A
(eds.) (CSHL Press, New York).

4. Jaiswal, JK, and Simon, SM. (2003) Total Internal Reflection Fluorescence Microscopy for
High Resolution Imaging of Cell Surface Events. Current Protocols in Cell Biology; Unit 4.12.
Jennifer Lippincott-Schwartz (ed.) (John Wiley and Sons Inc., New Jersey).