Dr. Gretchen Mahler
Department of Biomedical Engineering
Binghamton University, New York, USA
Hangout Title: "Development of physiologically realistic cell culture models of barrier tissues"
Hangout Schedule: March 16th: 8.30 am EST, 7.30 am CST, 5.30 am PST, 7 pm IST
Our lab uses microfluidics and 3D scaffolds to create physiologically realistic cell culture models of organs and tissues. These in vitro models can be engineered to signal cells using biochemical and biomechanical pathways, help to minimize animal testing, and provide tightly controlled, reproducible experimental conditions. The goal of our work is to replicate some of the cell-cell and cell-extracellular matrix interactions not easily studied in vivo or in silico to better understand how cells behave during disease and respond to chemicals or drugs. Our current research has applications toward cardiovascular disease and cancer and focuses on how disruptions in a tissue's local mechanical environment can lead to changes in cell phenotype and disease initiation and progression.
Postdoctoral Fellow-Biomedical Engineering, Cornell University, Ithaca, NY, 2008-2011
Ph.D.-Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, 2008
B.S.-Chemical Engineering, University of Massachusetts Amherst, Amherst, MA, 2002
Assistant Professor- Bioengineering, Binghamton University, Binghamton, NY, 2011-present
Honors & Awards
2008 The Hartwell Foundation Postdoctoral Fellowship
2007 Cornell University Department of Chemical and Biomolecular Engineering Hooey Prize
2002 University of Massachusetts Amherst Senior Leadership Award
2002 UMass Alumni Association Research Scholarship
2001 Tau Beta Pi Honor Society
2000 Hamilton Sundstrand Summer Internship Award
1.Mahler GJ, Frendl CM, Cao Q, and Butcher JB. Effects of shear stress pattern and magnitude on mesenchymal transformation and invasion of aortic valve endothelial cells. Biotechnology and Bioengineering. 2014. In press.
2. Mahler GJ. Metabolic Engineering: Enzyme control on a chip. Nature Nanotechnology. 2014; (9): 571 – 572.
3. Esch MB, Mahler GJ, Stokol T, and Shuler ML. Body-on-a-chip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury. Lab on a Chip. 2014; (14): 3081–3092.
4. Mahler GJ, Farrar EJ and Butcher JB. Inflammatory cytokines promote mesenchymal transformation in embryonic and adult valve endothelial cells. Arteriosclerosis, Thrombosis, and Vascular Biology. 2013; 33(1): 121-30.
5. Mahler GJ, Esch MB, Tako E, Southard TL, Archer SD, Glahn RP, and Shuler, ML. Oral exposure to polystyrene nanoparticles affects iron absorption. Nature Nanotechnology. 2012; 7(4): 264-270.
6. Mahler GJ and Butcher JB. Cardiac developmental toxicity. Birth Defects Research Part C: Embryo Today: Reviews. 2011; 93: 291-297.
7. Mahler GJ and Butcher JB. Inflammatory regulation of valvular remodeling: the good, the bad, and the ugly. International Journal of Inflammation. 2011; Article ID 721419, doi: 10.4061/2011/721419.
8. Butcher JB, Mahler GJ, and Hockaday LA. Aortic valve disease and treatment: The need for naturally engineered solutions. Advanced Drug Delivery Reviews. 2011; 63(4-5): 242-268.
9. Mahler GJ, Gould RA, and Butcher JT. Isolation and culture of avian embryonic valvular progenitor cells. Journal of Visualized Experiments. 2010; 44:
http://www.jove.com/index/details.stp?id=2159, doi: 10.3791/2159.
10. Chiu YN, Norris RA, Mahler GJ, Gould RA, Recknagel AK, and Butcher JB. Complementary and redundant roles for TGF3, BMP2, and VEGFA in embryonic atrioventricular valve remodeling. Tissue Engineering Part A. 2010; 16(11): 3375-3383.
11. Mahler GJ, Esch MB, Glahn RP, and Shuler ML. Characterization of a gastrointestinal tract microscale cell culture analog used to predict drug toxicity. Biotechnology and Bioengineering. 2009; 104(1): 193-205.
12. Mahler GJ, Shuler ML, and Glahn, RP. Characterization of Caco-2 and HT29-MTX cocultures in an in vitro digestion/cell culture model used to predict iron bioavailability. Journal of Nutritional Biochemistry. 2009; 20(7): 494-502.
13. McAuliffe GJ, Chang JY, Glahn RP, and Shuler ML. Development of a gastrointestinal tract microscale cell culture analog to predict drug transport. Molecular and Cellular Biomechanics. 2008; 5(2):119-32.
14. Shuler ML, McAuliffe GJ, and Tatosian DA. Animal surrogate systems for toxicity testing In Encyclopedia of Biomaterials and Biomedical Engineering. G. Wnek and G. Bowlin, Eds. New York: Marcel Dekker, Inc., March 27, 2006. 1-10.
15. McAuliffe GJ, Roberts L, and Roberts S. The influence of environmental conditions on the encapsulation of HepG2 liver cells in alginate. Journal of Undergraduate Research in Bioengineering. 2003; 3(1): 70-75.
16. McAuliffe GJ, Roberts L, Roberts S. Paclitaxel administration and its effects on clinically relevant human cancer and noncancer cell lines. Biotechnology Letters. 2002; 24(12): 959-964.