Arrhythmogenesis during heart failure is a major clinical problem. Regional electrical gradients produce arrhythmias, and cellular ionic transmembrane gradients are its originators.
Researchers investigated whether the nanoscale mechanosensitive properties of cardiomyocytes from failing hearts have a bearing upon the initiation of abnormal electrical activity.
Hydrojets through a nanopipette indent specific locations on the sarcolemma and initiate intracellular calcium release in both healthy and heart failure cardiomyocytes, as well as in human failing cardiomyocytes.
In healthy cells, calcium is locally confined, whereas in failing cardiomyocytes, calcium propagates. Heart failure progressively stiffens the membrane and displaces sub-sarcolemmal mitochondria.
Colchicine in healthy cells mimics the failing condition by stiffening the cells, disrupting microtubules, shifting mitochondria, and causing calcium release.
Uncoupling the mitochondrial proton gradient abolished calcium initiation in both failing and colchicine-treated cells.
Authors propose the disruption of microtubule-dependent mitochondrial mechanosensor microdomains as a mechanism for abnormal calcium release in failing heart.
http://www.cell.com/cell-reports/abstract/S2211-1247(15)01427-8
Edited
Latest News
Key mechanism controlling skin regeneration
Dynamic control of visually guided locomotion through corticosubthalamic projections
Vitamin K cycle is a potent ferroptosis suppressor
Cool room temperature inhibited cancer growth in mice
α-Synuclein fibril-specific nanobody reduces prion-like α-synuclein spreading in mice
Other Top Stories
Organelle distribution mechanism during mitosis!
Role of alternative splicing proteins in dementia
A new bacterial protein involved in transcription elongation identified!
Autophagy linked to beneficial effects of enduring mild stress
Designer enzymes!
Protocols
An improved organotypic cell culture system to study tissue-resident macrophages ex vivo
Protocol for spike-triggered closed-loop auditory stimulation during sleep in patients with epilepsy
Spectral fiber photometry derives hemoglobin concentration changes for accurate measurement of fl…
A Microengineered Brain-Chip to Model Neuroinflammation in Humans
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide re…
Publications
Glioblastoma hijacks neuronal mechanisms for brain invasion
Gut commensals expand vitamin A metabolic capacity of the mammalian host
A sphingolipid-mTORC1 nutrient-sensing pathway regulates animal development by an intestinal pero…
CDK9 activity switch associated with AFF1 and HEXIM1 controls differentiation initiation from ep…
Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegia
Presentations
Hydrogels in Drug Delivery
Lipids
Cell biology of carbohydrate metabolism
RNA interference (RNAi)
RNA structure and functions
Posters
ASCO-2020-HEALTH SERVICES RESEARCH AND QUALITY IMPROVEMENT
ASCO-2020-HEAD AND NECK CANCER
ASCO-2020-GENITOURINARY CANCER–KIDNEY AND BLADDER
ASCO-2020-GENITOURINARY CANCER–PROSTATE, TESTICULAR, AND PENILE
ASCO-2020-GYNECOLOGIC CANCER