In the current study, the team got further clues that arginylation played a role in the different roles of PRPS1 and PRPS2 when they examined cells in which arginylation had been blocked by making the enzyme that adds arginine, ATE1, inoperative.
They found that these cells had problems with making purine nucleotides, the As and Gs of the DNA sequence. The genetically modified cells also made more serine and glycine amino acids than normal cells, a further sign that purine synthesis was impaired. Because both PRPS proteins are involved in purine nucleotide synthesis, they were clear candidates for being subject to regulation by arginylation.
When the team introduced arginylated forms of PRPS1 and PRPS2 into cells, they found that PRPS1 was unstable and quickly degraded, while PRPS2 survived arginylation.
The structure of PRPS1 mRNA is likely to slow down the process of translation, the researchers believe, perhaps making that protein vulnerable to degradation once it is arginylated. Because PRPS2 mRNA lacks the same obstructions to translation, it appears able to survive arginylation without being targeted for degradation.
Knowing that PRPS2, which has been implicated in driving cancer, is regulated by arginylation provides a potential “switch” for tuning that activity. And the importance of mRNA structure in how a protein is eventually treated could give another way of targeting PRPS2’s activity and turning it down to prevent the uncontrolled growth seen in malignancies.
Edited
Latest News
Brain hormone regulate both…
By newseditor
Posted 17 Mar
Blocking long non-coding RN…
By newseditor
Posted 17 Mar
Artificial intelligence and…
By newseditor
Posted 17 Mar
Blood-brain barrier protein…
By newseditor
Posted 17 Mar
Preventing heart attacks an…
By newseditor
Posted 17 Mar
Other Top Stories
Manipulating chromatin modifier to enhance crop yield
Read more
Evolutionary trajectory of pattern recognition receptors in plants
Read more
How plants respond to changing conditions
Read more
Nitrate negatively affects floral scent and nocturnal pollination
Read more
Controlling the direction of root growth
Read more
Protocols
Integration of Kupffer cell…
By newseditor
Posted 18 Mar
A mouse DRG genetic toolkit…
By newseditor
Posted 17 Mar
An optogenetic method for t…
By newseditor
Posted 13 Mar
Profiling native pulmonary…
By newseditor
Posted 08 Mar
Neuromuscular organoids mod…
By newseditor
Posted 06 Mar
Publications
Synaptopathy: presynaptic c…
By newseditor
Posted 18 Mar
Allergic Rhinitis
By newseditor
Posted 18 Mar
ALK upregulates POSTN and W…
By newseditor
Posted 18 Mar
PRODH safeguards human naiv…
By newseditor
Posted 18 Mar
Secretin-dependent signals…
By newseditor
Posted 17 Mar
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar