Biophysicists created a new fluorescent protein. Besides glowing when irradiated with ultraviolet and blue light, it is exceedingly small and stable under high temperatures. The authors of the paper, published in the journal Photochemical & Photobiological Sciences, believe the protein holds prospects for fluorescence microscopy. This technique is used in research on cancer, infectious diseases, and organ development, among other things.
Fluorescence microscopy is a method for studying living tissue that relies on induced luminescence. After being exposed to laser radiation at a particular wavelength, some proteins emit light at a different wavelength. This induced "glow" can be analyzed using a special microscope. Researchers append such fluorescent proteins to other proteins via genetic engineering to make the latter ones visible to the microscope and observe their behavior in cells. Fluorescence microscopy proved so scientifically valuable that one Nobel Prize was awarded for its discovery, followed by another one for radically improving the method's accuracy.
Up until now, the fluorescent proteins used for such observations had several flaws. They were vulnerable to heat, fairly bulky, and only glowed in the presence of oxygen.
"For one thing, our protein is more thermostable than its analogues: It only denaturates at 68 degrees Celsius," said the paper's lead author. "It is also miniature, while most of the currently used fluorescent proteins are rather bulky. On top of that, it can emit light in the absence of oxygen."
The team originally identified the protein with these remarkable properties in the cells of a the thermophilic bacterium -- that is, one which lives in high-temperature environments, such as hot springs. The researchers then genetically engineered a DNA sequence that reproduced the protein's fluorescent segment but not the other parts, which would make the molecule larger.
By introducing the gene that encodes the protein into the cells of another bacterium, Escherichia coli, the team turned it into a factory mass-producing the fluorescent protein with unique properties.
Researchers studying the processes that occur in living cells have been waiting for a protein combining these crucial features for a long time. By introducing it into cells, they can now obtain essential data on cell life and death. To name a few applications, fluorescence microscopy is seen as one of the best tools for investigating the mechanism behind malignant tumor genesis and development. It is also useful for research on cell signaling and organ development.
The proteins previously used in fluorescence microscopy were bulky and thermally unstable, putting limitations on the method. Thanks to the team, that obstacle has been eliminated.
https://mipt.ru/english/news/scientists_engineer_unique_glowing_protein
https://pubs.rsc.org/en/content/articlelanding/2019/PP/C9PP00067D#!divAbstract
Latest News
Wiring of the human neocortex
By newseditor
Posted 24 Apr
Abusive drugs hijack natura…
By newseditor
Posted 23 Apr
Mechanism of action of the…
By newseditor
Posted 23 Apr
Role of fat in rare neurolo…
By newseditor
Posted 23 Apr
How protein synthesis in de…
By newseditor
Posted 22 Apr
Other Top Stories
Laboratory technique recapitulates all stages of egg cell development
Read more
Babies' spatial reasoning predicts later math skills
Read more
Embryos with "heteroplasmy," or the presence of both maternal and p…
Read more
Immune cell involvement in brain development revealed!
Read more
Blood cell types emerge through internal struggles
Read more
Protocols
A programmable targeted pro…
By newseditor
Posted 23 Apr
MemPrep, a new technology f…
By newseditor
Posted 08 Apr
A tangible method to assess…
By newseditor
Posted 08 Apr
Stem cell-derived vessels-o…
By newseditor
Posted 06 Apr
Single-cell biclustering fo…
By newseditor
Posted 01 Apr
Publications
Harnessing gastrointestinal…
By newseditor
Posted 24 Apr
Sex-specific modulation of…
By newseditor
Posted 24 Apr
Exploiting pancreatic cance…
By newseditor
Posted 23 Apr
Structure of antiviral drug…
By newseditor
Posted 23 Apr
Type-I-interferon-responsiv…
By newseditor
Posted 23 Apr
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar