Two-step self-assembly in spider silk

They are lightweight, almost invisible, highly extensible and strong, and of course biodegradable: the threads spiders use to build their webs. In fact, spider silk belongs to the toughest fibres in nature. Based on its low weight it even supersedes high-tech threads like Kevlar or Carbon. Its unique combination of strength and extensibility renders it in particular attractive for industry. Whether in aviation industry, textile industry, or medicine - potential applications of this magnificent material are manifold.

Since long time material scientists continue to try reproducing the fibre in the laboratory, but with limited success. Today, it is possible to manufacture artificial spider silk of similar properties as the prototype, but the molecular-level structural details responsible for material properties await to be disclosed. Now, scientists delivered new insights. The results are published in the scientific journal Nature Communications.

"The silk fibres consist of protein building blocks, so-called spidroins, which are assembled by spiders within their spinning gland", explains the author. The terminal ends of building blocks take special roles in this process. The two ends of a spidroin are terminated by an N- and a C-terminal domain.

The domains at both ends connect protein building blocks. In the present study, researchers took a close look at the C-terminal domain. The C-terminal domain connects two spidroins through formation of an intertwined structure that resembles a molecular clamp. "We observed that the clamp self-assembles in two discrete steps. While the first step comprises association of two chain ends, the second step involves the folding of labile helices in the periphery of the domain," that author said.

This two-step process of self-assembly was previously unknown and may contribute to extensibility of spider silk. It is known that stretching of spider silk is associated with unfolding of helix. Previous work, however, traced extensibility back to the unfolding of helices in the central segment of spidroins. "We propose that the C-terminal domain might also act as module that contributes to extensibility" explains the author.

In their study the researchers investigated protein building blocks of the nursery web spider Euprosthenops australis. They used genetic engineering to exchange individual moieties of building blocks and modified the protein chemically using fluorescent dyes. Finally, the interaction of light with soluble proteins disclosed that the domain assembles in two discrete steps.

https://www.uni-wuerzburg.de/en/news-and-events/news/detail/news/molecular-insights-into-spider-silk/