Mitochondria, best known for their role as powerhouses of eukaryotic cells, fulfill numerous vital functions. Knowledge about the precise protein composition as well as the functions of individual proteins is essential to understand fundamental processes of cell biology and diseases that are caused by cellular defects.

A team of scientists discovered, counted and determined new mitochondrial proteins with unknown function in the model organism baker's yeast. The study will serve as a source of information for researchers interested in the biology of mitochondria - from yeast to human subjects. The study was recently published in the journal Cell Reports.

Using 'quantitative mass spectrometry' and bioinformatics methods, the research group first determined the abundance of thousands of proteins in different cellular fractions of baker's yeast. They then analyzed mitochondrial proteins using biochemical methods and microscopy. The resulting mitochondrial proteome comprises a total of 901 proteins, including 82 proteins not previously associated with mitochondria. For an additional 119 a mitochondrial localization had been ambiguous.

While humans require oxygen to breathe, yeast cells can either consume oxygen or use a different metabolic pathway called fermentation, a process well known for producing alcoholic beverages. The researchers cultured yeast cells in a fermentative or respiratory medium and determined that the shift from fermentative to respiratory conditions caused dramatic changes in the mitochondria: The amount of mitochondrial proteins in a single cell doubles and the enzymes required for respiration are even four times more abundant when growing in a respiratory medium.

The scientists further studied in which areas of the mitochondrion the various proteins are localized and how newly discovered proteins interact with other proteins in a network. Through this study the researchers have gained extensive data about the newly defined mitochondrial proteome of baker's yeast.

http://www.pr.uni-freiburg.de/pm-en/2017/discovering-counting-cataloguing-proteins?set_language=en

http://www.cell.com/cell-reports/abstract/S2211-1247(17)30811-2