Circulating tumor DNA (ctDNA) from CSF for characterizing and real time tracking of childhood brain cancer  

Circulating tumor DNA (ctDNA) from CSF for characterizing & real time tracking of childhood brain cancer  

Building on previous research latest findings from a proof-of-concept study published in Nature Communications, show that the analysis of cerebrospinal fluid (CSF) circulating tumor DNA (ctDNA), allows for the more precise characterization, molecular diagnosis (including subtyping and risk stratification), and real time tracking of medulloblastoma (MB) - the most prevalent malignant brain tumor in childhood.

The results also evidence that this novel and less invasive approach can also provide precious insights into minimal residual disease, tumor evolution, and identify disease at relapse.

While the genomic characterization of medulloblastoma promises a more precise molecular diagnosis, accessing tumor tissue -due to the very nature of its location- is complex and can suppose high-risk surgical procedures and stereotactic biopsies, neither of which can guarantee access to a truly representative part of the tumor. The classification of this tumor type is also particularly challenging due to its ever changing intra-tumor heterogeneity which demands an in-depth and serial analysis of the evolving genomic landscapes in response to treatment or during disease recurrence. Establishing the molecular make-up of medulloblastoma is particularly critical since this tumor type is the most common of all paediatric brain cancers.

"Results from this present study represent a potentially practice-changing approach in more precisely steering the surgical and clinical management of these young patients. By evidencing the promise of cerebrospinal fluid as liquid biopsy for the personalized treatment of this extremely aggressive disease, our findings could ultimately spur the development of more effective and tailored therapeutic strategies. As importantly, they could also reduce excessive treatment to prevent long-term secondary effects," observes corresponding author.

Hydrocephalus is an abnormal accumulation of cerebrospinal fluid and is very common in paediatric patients whose tumors are growing in the area of the posterior fossa, including medulloblastoma. In these cases, cerebrospinal fluid drainage must be performed prior to surgery to reduce increased intracranial pressure (ICP). Since the fluid is routinely collected for cytologic evaluation, this facilitates easy access to samples for research in the laboratory.

Data derived from the liquid biopsy is therefore fundamental in disease prognosis as well as gauging tumor aggressiveness. This information is crucial for both the surgeon and the oncologist when balancing the risks and benefits of each intervention and therapeutic strategy.

The study investigators also show that CSF ctDNA facilitates the subgrouping and risk stratification of patients suffering from medulloblastoma, which provides valuable insights for the diagnosis and prognosis of disease towards more effectively matching therapies to the unique specificities of each individual patient. Specifically, certain genetic mutations are critical in treatment selection, such as mutations in the PTCH1 gene which encodes a protein called patched-1. In this case, inhibitors of the Hedgehog signaling pathway could be a more effective treatment option.
"Our research represents an important forward step towards better characterizing medulloblastoma, providing an earlier and more accurate molecular diagnosis, more closely monitoring our patients, and providing crucial data on minimal residual disease tumor evolution and disease at relapse. Larger cohort studies are now warranted to bring cerebrospinal fluid as liquid biopsy closer to the clinic and deliver on the promise of precision medicine for the treatment of this disease," concludes the senior author.