Scientists have discovered a fast, noninvasive method that could lead to the early diagnosis of colorectal cancer.
Using ultrasensitive, high-speed technology, the researchers identified a suite of molecules in the feces of mice that signifies the presence of precancerous polyps.
This "metabolic fingerprint" matches changes in both mouse and human colon tumor tissues and suggests a potential new diagnostic tool for early detection of colorectal cancer in a clinical setting.
The findings were reported in the Journal of Proteome Research.
Though early detection is key to successful treatment, most screening tests are limited in diagnostic capability or ease of application. Colonoscopy, for example, is a known life saver but is costly and unappealing to many people who might otherwise undergo testing.
Decreasing the invasiveness of the procedure could help. More people would be willing to provide a stool sample than undergo a biopsy through a colonoscope. In addition, colonoscopes can only extend a limited distance into the large intestine, potentially missing some polyps.
Researchers discovered the molecular fingerprint for colon cancer using a technology called ion mobility-mass spectrometry. IMMS is found in sensor devices worldwide that sniff out illicit drugs, chemical warfare agents and explosives in airports. In this case, IMMS was coupled with ultraperformance liquid chromatography.
The researchers first identified metabolic products from normal colon tissue in both humans and mice. IMMS can measure hundreds of metabolites simultaneously, such as enzymes, fats, glucose and amino acids.
The scientists then compared this normal profile to that found in cancerous colon tissues from humans and research mice with polyps in their colons that mimic those in humans.
In both cases, the scientists found that colon cancer caused significant changes in fat metabolism, especially for lipids and fatty acids. These abnormalities created a molecular fingerprint that was similar in humans and mice, said Hill.
Next, they examined droppings from transgenic and control mice to see if the molecular fingerprint could be found in feces as well.
Indeed IMMS detected many of the same metabolic abnormalities seen in the previous study and could clearly distinguish between healthy mice and those with colorectal cancer.
"The feces was not exactly the same as the tissue samples, but it had a lot of similarities to the tissue," said the author. "We found the lipids and fatty acids were changing—and there were also changes in the amino acids."
Specifically, an important class of fats called lysophospholipids changed dramatically, said the author.
"These types of lipids are known to be important in the development of cancer and are particularly tied to colorectal cancer."
"The benefit of early detection is that we can catch cancer before it metastasizes to other parts of the body," said the author. "Our results represent the zero stage of cancer, the polyp stage - as early as colon cancer can be detected."
Their next step, if funded, is to evaluate human stool samples to see if the molecular fingerprint is present with colorectal cancer in people.
Fat in feces points to early presence of colorectal cancer
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