For a cell in an embryo, the secret to becoming part of the baby's body instead of the placenta is to contract more and carry on dancing, scientists have found. The study, published today in Nature, could one day have implications for assisted reproduction.
After a sperm cell fertilizes an egg cell, that fertilized egg divides repeatedly, forming a ball of cells. Shortly before the embryo implants in the womb, some of those cells move inwards. These are the cells that will develop into all the baby's body parts. The cells left on the surface will become the placenta, connecting the embryo to the mother's uterus.
Researchers found that, in a mouse embryo, whether a cell moves to the middle or stays on the surface depends on how strongly it can contract. By combining computer modelling and experiments in the lab, the scientists determined that cells that contract at least one-and-a-half times more strongly than their neighbors move inwards.
But why do some cells contract more than others? The answer, the scientists found, is that they receive unequal inheritances: different amounts of apical proteins, molecules that stop the cell from contracting. Cells that inherit small amounts of apical proteins contract more, and move inwards to give rise to the embryo. By contrast, cells with large amounts of apical proteins contract less and stay on the surface, becoming the placenta. But when the scientists prevented all cells from contracting, they were surprised at the result.
"We found that if you interfere with contractility, the cells stay at the surface. But instead of becoming placenta cells, as you'd expect, they become embryonic," says one of the authors. "This tells us that sensing the forces around them may be important for cells to know where they are, and what to become."
Researchers previously discovered that in the first few days of an embryo's life, all its cells perform a little 'dance', as a wave of contraction sweeps around their surface. The scientists now found that when the embryo is 3 to 4 days old, the cells that will become the placenta no longer dance.
Although the study looked at mouse embryos, the first stages of development are similar in mouse and human embryos, so the researchers expect their findings to hold true for our own species. If so, they could one day be relevant for researchers and clinicians doing pre-implantation diagnostic tests on in vitro fertilised (IVF) embryos. Screening IVF embryos for genetic disorders before implanting them in the mother entails taking a cell from the embryo, to analyze its DNA. Knowing that cells which will become the placenta don't dance may help clinicians to pick either placental or embryonic cells to test.
"We now understand a lot more about the mechanical aspect of how cells move inwards and become the embryo, but it's also raised a lot of questions about how that is translated into gene expression," says the author. "We're also looking at other factors that could be involved, like how cells communicate, and sense contacts with each other."
Strength of contraction determines whether cells become embryo or placenta
- 1,405 views