Scientists demonstrated that substituting a single nucleotide in the gene coding for the nicotinic acetylcholine receptor can lead to functional changes in airway cells and result in symptoms similar to COPD, independent of smoking. The scientists moreover identified the molecular mechanisms involved in this pathological phenomenon. The results of this research were published in the journal Nature Communications .

Previous genetic studies conducted in humans initially linked this disease to genes located on chromosome 15 coding for subunits of the nicotinic acetylcholine receptor (nAChR). In a new study, scientists focused their research on the variability of a single nucleotide (an adenine base rather than a guanine base) in the CHRNA5 gene, coding for the alpha5 subunit of the nAChR receptor, on chromosome 15. This version of the gene occurs in the genomes of 37% of the European population. The scientists examined the effects of this variation on airway cells.

In the animal model carrying this version of the gene, the study revealed the onset of emphysema destroying the pulmonary alveolar walls, a phenomenon observed in COPD patients. "We revealed the biological impact of a mutation in a gene linked to COPD," comments the studies joint last author. The scientists established a connection between the expression of this gene version and airway epithelium inflammation and remodeling. Functional impairment was observed in progenitor airway basal cells located in the deep layer of the epithelium and responsible for epithelial renewal. The scientists observed this dysfunction particularly during scarring of the bronchial epithelium.

The study findings were subsequently confirmed in humans. Histological analysis of cells from samples of nasal polyps from 123 non-smoking patients at Reims University Hospital also revealed a link between epithelial remodeling and this gene mutation. "This gene version appears to be a genetic predisposing factor for the disease independent of smoking. COPD onset then occurs subsequent to repeated lesions of airway tissue caused by other molecules including atmospheric pollutants," comments studies other joint last author.

Moreover, the signaling pathways involved were identified through in vitro analysis of animal cells expressing the modified version of the nicotinic receptor.

https://www.nature.com/articles/s41467-021-26637-6

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