Scientists investigating heart failure have been limited to studying diseased heart tissue in the lab -- understandably, as people don't tend to pluck out a healthy heart for the sake of research. But now, scientists with access to unusable, yet still healthy, donor hearts have been able to investigate the genomic pillars behind the transition from healthy hearts to heart failure.
In doing so, researchers have created one of the first maps to reveal gene activity and connectivity as the heart shuts down. The authors call it a gene network. These networks are akin to social networks. "Let's say we traced the whereabouts of the human resources department at Stanford. We could see that they tend to park in the same area, go to the same office, and get lunch in the same place," the author said. "They move together, and so it can be reasonably inferred that they are somehow related to each other."
Tracing a gene network for heart failure is like that, only instead of watching physical movements, the authors watched for changes in gene expression, paying attention to how it changes as healthy hearts degenerates.
By delineating these gene networks, the group has discovered one gene in particular that seems to be at the center of the action. It appears to be highly connected in heart failure, meaning its activity is similar to that of many neighbors. What's even more exciting, the author said, is that when the researchers disabled the function of this gene in mouse models of heart failure, the mice were protected and did not succumb to the cardiac condition. A paper providing details of the study is published in Nature Communications.
Heart failure is not one simple condition, said the senior author. It's more like an umbrella term that describes the heart's inability to pump blood regardless of the cause. "It could be a heart attack, a genetic cause, high blood pressure, a valve problem or something else entirely," the author said. "Regardless of how the heart deteriorates, we believe there's one final, common pathway that ultimately leads to heart failure."
Now, the web of genes that they've mapped is providing new insight into how that pathway unfolds, and which genes are crucial to its activation.
"Maybe someday we'll be able to peer into a cell and watch as the networks are actively changing in real time," the senior author said. "But right now what we have is human tissue that's sort of frozen at a moment in time, and so we can use that to look at which genes are involved in this process."
The group took tissue samples from more than 300 hearts (half from hearts removed from patients with heart failure who were getting heart transplants and half from healthy donor hearts) and ran genomic tests to determine gene expression activity. It was essential, to have the healthy donor hearts available. "Sometimes, for logistical reasons, a donor's heart isn't usable for transplantation, so instead of wasting these organs, we repurposed them for our study." Thanks to a dedicated, multi-institutional surgical team that worked around-the-clock -- sometimes venturing out in the wee hours of night -- the group secured more than 100 healthy hearts.
To date, studies that compare the genomics of healthy hearts and heart failure have taken place mainly in mice. But with the healthy donor heart tissue, the team was able to compare and contrast genomic information in human hearts. They found that fewer biological pathways were involved in heart failure compared with healthy hearts, but there were more genes involved in those pathways. It's almost as if the cells were "focusing" their efforts, presumably as part of a last-ditch effort to restabilize the declining heart, said the senior author.
Perhaps most telling, PPP1R3A had one of the biggest leaps in gene connectivity during the transition to heart failure, meaning it became associated with the activity of many other genes. And although it hasn't been implicated in heart failure in the past, its role does seem to fit with some of the symptoms, the senior author said. The heart's energy source typically comes from fatty acids, but it switches to glucose when it goes into failure. PPP1R3A as a central regulator makes sense because the gene plays a critical role in the metabolism of glucose within cells, the senior author said.
What's more, the same networks that pointed researchers to PPP1R3A also turned up dozens of other new gene interactions during heartfailure.
The authors confirmed PPP1R3A's causal role in heart failure by testing the effect of the gene -- or lack thereof -- in mouse models of high blood pressure. It turned out that mice lacking the PPP1R3A gene maintained normal heart function, whereas those with the gene succumbed to heart failure.
"Across the population, there are plenty of people with high blood pressure who never go into heart failure, and there are some that do," the senior author said. "We're sort of mimicking that in these mouse studies. If we were able to inhibit this gene somehow in humans, we could potentially have a therapeutic drug that could protect patients from heart failure."
http://med.stanford.edu/news/all-news/2019/06/gene-networks-reveal-transition-from-healthy-to-failing-heart.html
https://www.nature.com/articles/s41467-019-10591-5
http://sciencemission.com/site/index.php?page=news&type=view&id=publications%2Fpathologic-gene-network&filter=22
Latest News
Metabolic rewiring promotes…
By newseditor
Posted 18 Apr
A drug to prevent flu-induc…
By newseditor
Posted 18 Apr
New origin of deep brain waves
By newseditor
Posted 17 Apr
Starving cells hijack prote…
By newseditor
Posted 17 Apr
Miniature battery-free epid…
By newseditor
Posted 17 Apr
Other Top Stories
Mature heart muscle cells created in the laboratory from stem cells
Read more
Cell of origin affects malignancy and drug sensitivity of brain tumors
Read more
Protein essential for healthy gut cell development identified!
Read more
Reprogramming of liver cells to pancreas progenitor cells based on…
Read more
The intestine has a reservoir of stem cells that are resistant to c…
Read more
Protocols
MemPrep, a new technology f…
By newseditor
Posted 08 Apr
A tangible method to assess…
By newseditor
Posted 08 Apr
Stem cell-derived vessels-o…
By newseditor
Posted 06 Apr
Single-cell biclustering fo…
By newseditor
Posted 01 Apr
Modular dual-color BiAD sen…
By newseditor
Posted 31 Mar
Publications
How does the microbiota con…
By newseditor
Posted 18 Apr
The integrated stress respo…
By newseditor
Posted 18 Apr
The immunobiology of herpes…
By newseditor
Posted 17 Apr
Circulating microbiome DNA…
By newseditor
Posted 17 Apr
Spindle oscillations in com…
By newseditor
Posted 17 Apr
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar