Structural changes in G6PD causes common blood disorder

Structural changes in G6PD causes common blood disorder


With a name like glucose-6-phosphate dehydrogenase deficiency, one would think it is a rare and obscure medical condition, but that's far from the truth. Roughly 400 million people worldwide live with potential of blood disorders due to the enzyme deficiency. While some people are asymptomatic, others suffer from jaundice, ruptured red blood cells and, in the worst cases, kidney failure.

Now, a team of researchers have uncovered the elusive mechanism behind the most severe cases of the disease: a broken chain of amino acids that warps the shape of the condition's namesake protein, G6PD. The team reports their findings in Proceedings of the National Academy of Sciences.

G6PD's role in our health is hard to overstate. In red blood cells, which deliver oxygen to other cells, the enzyme helps remove more chemically reactive and potentially harmful oxygen molecules, such as hydrogen peroxide, and convert them to water and other more inert byproducts. If the G6PD protein isn't working properly, red blood cells also stop working properly and can, in the worst cases, rupture.

Still, doctors have mostly been at a loss to treat the disease, especially in the most severe cases, known as Class I. "At the moment the treatment for these patients is blood transfusions," said a senior author on the new study.

Medical researchers have known for decades that upwards of 190 different mutations can lead to some forms of G6PD deficiency, and they also worked out the structure of ordinary G6PD in various forms decades ago. The authors have also made strides in identifying drugs that may treat some less-severe forms of the disease, known as Class II and III.

Unfortunately, those drugs do not work for Class I cases, which have proved especially confusing. Most of the mutations that lead to the most severe symptoms were known to occur in areas far away from the active site where it binds to substrate molecules for its enzyme reactions - the usual spot where such mutations would be thought to cause problems.

To better understand what was going on, the authors started with X-ray crystallography studies of four forms of G6PD associated with the worst G6PD-deficiency symptoms.

The team found that even though the mutations are far from G6PD's active site, they break up connections in a chain of amino acids that help stabilize the protein. That has a kind of domino effect on the whole protein molecule: It bends awkwardly, and a molecular arm that should help molecules bind to G6PD's active site instead wobbles unpredictably around. The team confirmed aspects of those results with a mixture of other techniques, including small angle X-ray scattering at Beam Line 4-2 at SSRL, computer simulations and cryogenic electron microscopy.

The results show for the first time how the most severe cases of G6PD deficiency work at the molecular level and could help researchers design new drugs to treat the disease.

https://www6.slac.stanford.edu/news/2021-01-19-researchers-discover-long-sought-mechanism-behind-worst-cases-common-blood-disorder

https://www.pnas.org/content/118/4/e2022790118

http://sciencemission.com/site/index.php?page=news&type=view&id=publications%2Flong-range-structural&filter=22

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