From a medical point of view, there are different types of diabetes. Maturity onset diabetes of the young (MODY) is a rare monogenetic (caused by the inheritance of a single gene mutation) form of diabetes which accounts for 1-2% of diabetes cases. MODY3 is the most common form of monogenic diabetes in the Caucasian population and it is caused by mutations in the transcription factor HNF1A. The patients progressively develop hyperglycemia, characterized by a high blood sugar level, due to a perturbed insulin secretion from the β cells. However, the pathogenesis, meaning how the disease develops, is still unknown.

Researchers used patient-derived stem cells to investigate why mutations in HNF1A progressively lead to diabetes in MODY3. The scientists identified a new pathogenic mechanism for diabetes onset in MODY3. The studied MODY3 mutation caused hypersecretion of insulin from β cells. A key finding, that will contribute towards preventing the mutation carriers to become diabetic.

The phenotype of MODY3 patients is very heterogeneous, which is reflected by, among other things, a highly variable age at disease onset. Despite many efforts in trying to understand the underlying disease mechanism of MODY3, the determinants of disease onset are poorly understood. A better understanding of what triggers diabetes in MODY3 patients opens up for targeted treatments that delay or even prevent the disease.

Using patient-specific induced pluripotent stem cells (iPSCs), the authors recapitulated the insulin secretion sensitivity to the membrane depolarizing agent sulfonylurea, a phenomenon commonly observed in MODY3 patients. Unexpectedly, MODY3 patient-specific HNF1A+/R272C β cells showed an excessive secretion of insulin both in vitro and in vivo after transplantation into mice.

Consistently, a trend of increased birth weight in human HNF1A mutation carriers compared to healthy siblings was identified. Reduced expression of potassium channels, specifically the KATP channel, in MODY3 β cells, increased calcium signaling. In addition, the rescue of the insulin hypersecretion phenotype by pharmacological targeting ATP-sensitive potassium channels or low voltage-activated calcium channels, suggest that more efficient membrane depolarization underlies the hypersecretion of insulin in MODY3 β cells.

The study highlights the importance of patient-specific iPSCs as a platform for studying early disease mechanisms that pave the way for personalized medicine. The results emphasize the importance of early identification of hyperinsulinemia in HNF1A mutation carriers. Hyperinsulinemia is a condition with abnormally high concentration of insulin in the blood. This leads to reduced blood sugar levels, which if too low may be life-threatening.  

With this knowledge, the researchers paved the way for further investigations to test if treatments to prevent hyperinsulinemia – such as diets or drugs – in newborns carrying HNF1A mutations will delay or even prevent the onset of MODY3 diabetes later in life.

https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00485-4

http://sciencemission.com/site/index.php?page=news&type=view&id=publications%2Fan-insulin-hypersecretio&filter=22