A distinctive pattern of epigenetic changes in insufficient sleep

An insufficient amount of sleep and qualitatively poor sleep are common among the general population. Prior studies have indicated that prolonged insufficient sleep and poor sleep quality are associated with a heightened risk of cardiovascular diseases, dementia and psychiatric disorders, particularly depression, yet the molecular mechanisms underlying this connection are not well known.

In a study men between 25 and 55 years of age suffering from insufficient sleep were compared to healthy men who get enough sleep. Altogether 105 men took part in the study.'

DNA was extracted from blood leukocytes collected from the study subjects, to determine the methylation levels of nearly half a million CpG bases. DNA methylation is among what are known as epigenetic mechanisms, impacting gene expression and, thus, the functioning of cells and tissues.

"Based on our findings, no single DNA methylation element significantly differed between those suffering from a lack of sleep and healthy control subjects. However, the analysis of molecular networks revealed that insufficient sleep resulted in changes in DNA methylation in the gene regulatory elements belonging to the nervous system development pathway," explains the senior author of the study. The authors found that 78% of the DMPs (differentially methylated positions) were hypomethylated in cases in both cohorts, suggesting that insufficient sleep may be associated with loss of DNA methylation.'

Changes were also observed in a genomic region that has earlier been associated with the hereditary Smith-Magenis syndrome. Inverse circadian rhythm is among the potential symptoms of this disease.

The findings indicate that lack of sleep is associated with systemic DNA alterations that affect the genome.

"We are currently looking into how permanent these changes are. In addition, we are interested in how they are linked with the long-term health risks of sleep deprivation," the senior author says.

"The findings require further studies, and they must be repeated with other datasets," stresses the first author of the article published in the Scientific Reports journal.