As every cook has experienced: When balsamic vinegar and olive oil are mixed, both liquids separate. Round vinegar drops form, which then float on the surface of the oil. In physical terms, this constitutes the formation of two phases in the liquid. Phase separation of molecules also takes place inside cells. Here, liquid drops form in the cell plasma.
Researchers have now discovered that a class of enzymes - which are dual specificity kinases - actively control this process in cells. When a cell divides, the enzyme DYRK3 promotes the mixing of the phases. Authors show that DYRK3 kinase activity is essential to prevent the unmixing of the mitotic cytoplasm into aberrant liquid-like hybrid organelles and the over-nucleation of spindle bodies. This guarantees that the cells can correctly build the machinery for separating the chromosomes and dividing the cell content. After division, the enzyme is broken down and the individual phases start to form again. If everything goes according to plan, the genetic material, organelles and cell contents are correctly distributed among the daughter cells. "These fundamental findings give us completely new insights into cell division: as a process in which the cell contents mix together and then separate again," says the author.
The knowledge that this physico-chemical process in cells is actively regulated by enzymes is highly relevant to the research into and treatment of various wide-spread diseases. If phase separation during cell division does not work properly, the separation of the chromosomes is incomplete and they are then incorrectly distributed to the daughter cells - a significant characteristic of numerous kinds of cancer. Many protein defects that possibly cause neurodegenerative problems are also presumably the consequence of a failed intracellular phase separation. "Thanks to the discovery as to which proteins control phase separation, new strategies can be pursued to prevent mistakes in this process," says the author.
Both the distribution of the cell contents across the daughter cells and the specific retention of "old" components in a cell are important processes in aging. In this case, as well, a finely controlled mixing and separating of phases is an important process that determines the fate of cells. A further possible application would be viral infections: When viruses infect cells, they often trigger the phase separation of molecules. In this way, they create isolated areas in which new viruses can form. "Since we now know that these enzymes control intracellular phase separation during viral infections, we can research new antiviral therapies," says the author.
http://www.media.uzh.ch/en/Press-Releases/2018/cell-division-control.html
https://www.nature.com/articles/s41586-018-0279-8
Latest News
Brain hormone regulate both…
By newseditor
Posted 17 Mar
Blocking long non-coding RN…
By newseditor
Posted 17 Mar
Artificial intelligence and…
By newseditor
Posted 17 Mar
Blood-brain barrier protein…
By newseditor
Posted 17 Mar
Preventing heart attacks an…
By newseditor
Posted 17 Mar
Other Top Stories
Loneliness is a heritable trait?
Read more
How memories form in real time
Read more
How brain separates relevant and irrelevant information
Read more
Link between chronic pain and lack of sleep
Read more
Smoking has long lasting impact on human genome
Read more
Protocols
Integration of Kupffer cell…
By newseditor
Posted 18 Mar
A mouse DRG genetic toolkit…
By newseditor
Posted 17 Mar
An optogenetic method for t…
By newseditor
Posted 13 Mar
Profiling native pulmonary…
By newseditor
Posted 08 Mar
Neuromuscular organoids mod…
By newseditor
Posted 06 Mar
Publications
Synaptopathy: presynaptic c…
By newseditor
Posted 18 Mar
Allergic Rhinitis
By newseditor
Posted 18 Mar
ALK upregulates POSTN and W…
By newseditor
Posted 18 Mar
PRODH safeguards human naiv…
By newseditor
Posted 18 Mar
Secretin-dependent signals…
By newseditor
Posted 17 Mar
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar