The current work shows that changes in the genetic code of a particular gene can control epigenetic marks at different genes, implying that a gene's genetic code can affect whether other genes are turned on and off, which makes it important to understand the function of all genes involved, not just the one with the so-called misspelling. "Our findings suggest that looking only at genes with misspellings related to autism might be too narrow a focus," says senior author. "Instead of looking solely at the genes directly implicated in autism through their genetic code changes, we really should be also studying the functions of the other genes implicated by these changes in genetic code through epigenetic connections."
Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by deficits in socialization, verbal and nonverbal communication, and repetitive behaviors. Since the 1960s, the prevalence rates have skyrocketed, with one in 68 U.S. children now being diagnosed with ASD, according to the Centers for Disease Control and Prevention.
While a few rare gene variants can explain a fraction of ASD cases, researchers suspected that the epigenome could add significantly more to understanding this disease.
To investigate this question, they started by surveying four different tissue types--blood and cord blood from their own collections, as well as lung and fetal brain tissue from public collections--to find small variations in the genetic code of each sample that appear to be responsible for DNA methylation state, a type of epigenetic modification, in that particular tissue.
The researchers examined millions of these genetic code variations, known as single nucleotide polymorphisms (SNPs) and found thousands that control DNA methylation in some or all tissue types. They then matched up these SNPs with those already known to be associated with autism and saw that more autism-associated genes act to control DNA methylation than would be expected by chance. This was true in both blood and fetal brain tissue.
When the researchers looked at the role of genes that were methylated by genes with code differences related to ASD, including additional genes beyond those with direct code changes, they found that the majority were involved in biological pathways that were important in immune function.
The finding wasn't surprising, senior author explains--numerous studies have identified abnormal gene expression of immune genes in autism samples and environmental experiences such as prenatal infection or prenatal exposure to pollutants that can ramp up immune responses are risk factors for ASD. However, none of the genetic code mutations directly identified in autism had pointed to these pathways. It is only when considering which other genes they may regulate that this biology is revealed.
More study on these biological pathways, could lead to specific genes or proteins that could be modifiable with pharmaceuticals or other interventions, possibly offering new ways to prevent or treat ASD.
"We made our findings here by comparing brain data to blood data," author says, "but the vast majority we could have learned from blood."
Autism genes affect epigenetics of other genes!
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