Optogenetics is an elegant technique of controlling neurons in the brain using light. Neurons are loaded with special light-sensitive proteins called opsins that sense light (in the visible range), convert it into electric signals, and activate neurons. Conventionally, optical fibers connected to a light source are invasively implanted into the target tissue. But reaching the deep areas of the brain using this technique is usually accompanied by extensive tissue damage, light toxicity, and harmful effects of thermal irradiation. To reduce this damage, scientists use microparticles that emit visible light in response to near-infrared irradiation (NIR). These particles are injected into the target tissue and neuron activation is achieved without tissue damage. However, NIR has its limitations—it can only penetrate some millimeters of tissue.
Now, researchers have finally overcome this challenge by using X-rays to penetrate deep regions of the brain. The findings of this multidisciplinary study, was published in Nature Communications. “This new technology enables remote control of brain functions in living animals without damaging radio-sensitive cells in the body.”
X-rays are widely used for imaging the human body because of their penetrative capacity. Using this ability of the X-rays, the researchers could reach the deeper areas of the brain. However, opsins do not respond to X-rays. So, to convert the radiation into visible light, the researchers chose materials called scintillators. The senior author explains, “Scintillators, which emit visible light when irradiated with X-rays, have widely been used in X-ray inspection machines and CT scans, but their applications in biology have been limited. This work is the first application of scintillators to behavioral neuroscience.”
The researchers crushed yellow-emitting inorganic scintillator crystals synthesized in the lab and injected the microparticles into mice brain cells. When X-rays were irradiated onto a dissected mouse head, they passed through skin, skull, and brain tissue. In response to the X-rays, the microparticles emitted yellow light which activated the opsins for excitation and inhibition of neurons. The researchers were able to confirm these results in live mice.
To explore further, the researchers also tested whether the control of neural function by X-rays and opsins could induce a change in behavior. For this, they performed a conditioned place preference test in which mice were given a choice between two compartments, one out of which was exposed to X-ray radiation. The mice either had excitatory opsins or inhibitory opsins in neurons that govern this type of behavior. Before X-ray irradiation, no mice showed any preference for a compartment. However, after X-ray irradiation, mice with excitatory opsins had an increased preference for the X-ray irradiated compartment and mice with inhibitory opsins showed the opposite behavior.
Scintillator-based optogenetics has many potential applications, from modulating neurons for research to treatment of neurological disorders. The non-invasive nature of this technique allows experiments to be conducted without wires and plugs, not hindering observation and science. The world of neuroexploration thus seems to have found a new and wonderful probing technique!
Controlling neuronal function and behavior using X-rays
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