In a new study published in Proceedings of the National Academy of Sciences, researchers are the first to map the molecular structure and dynamics of an aggressive protein modification that spurs on Alzheimer's disease.
"Roughly ten percent of Alzheimer's disease cases are the result of identified mutations," says the senior author. "But 90 percent of Alzheimer's cases are not explained by these mutations, which is why we need to understand the molecular base of the disease."
Alzheimer's disease begins decades before the onset of symptoms. It starts the day microscopic, toxic protein fragments called beta amyloids (Aβ) glom onto each other. Those clumps form chains called fibrils, which band together to become a sticky, pleated sheet that builds on brain cells like plaque. As it accumulates, the plaque disrupts cell membranes and the communication between brain cells, causing them to die. Until now, understanding just the molecular makeup of the proteins - and the more aggressive subtypes that cause a rapid acceleration of the disease - has plagued researchers.
In this collaborative study, researchers targeted the structure and the dynamics of the aggressive, "seeding-prone" Ser-8-phosphorylated 40-residue Aβ (pS8-Aβ40) fibrils. They found that even when it existed in smaller amounts, pS8-Aβ40 acted as the alpha in structure polymorphism. It also had a higher level of cellular toxicity compared to other fibrils. In looking at the molecular structure, researchers found that the N-terminus, the creation point of the protein, played an important role in manipulating both the fibrils structures and the aggregation processes.
The pS8-Aβ40 fibril possesses strong cross-seeding ability to wild-type Aβ40 monomers, while the propagated fibrillar structure shows higher thermodynamic stability and core rigidity compared to the fibrils formed by the self-nucleation of wild-type Aβ40.
"Fibrils are very resilient to treatment that prevents aggregation," says the lead author. "Whatever you do to them in the test tubes, they adjust, find a way to go into a toxic state and aggregate."
The lead says mapping the structure of pS8-Aβ40 is just the first piece of a larger puzzle. The team plan do the same for several important protein modifications, focusing on the static structure, dynamics and stability of each. Eventually, this information might one day lead to ideas how to come up with drugs that can break the vicious cycle of cell degeneration.
https://www.cudenvertoday.org/identifying-the-molecular-structure-of-one-of-alzheimers-stickier-culprits/
https://www.pnas.org/content/early/2019/05/14/1818530116
Latest News
New origin of deep brain waves
By newseditor
Posted 17 Apr
Starving cells hijack prote…
By newseditor
Posted 17 Apr
Miniature battery-free epid…
By newseditor
Posted 17 Apr
Molecular causes of differe…
By newseditor
Posted 16 Apr
Cell's 'garbage disposal' h…
By newseditor
Posted 16 Apr
Other Top Stories
Tubular mitochondria network in Multiple Sclerosis microglia
Read more
Keeping the inflammation in check!
Read more
Controlling inflammation helps in lifespan extension and aging
Read more
Mediating eye-brain immunity
Read more
Why killer T cells lose energy inside of solid tumors
Read more
Protocols
MemPrep, a new technology f…
By newseditor
Posted 08 Apr
A tangible method to assess…
By newseditor
Posted 08 Apr
Stem cell-derived vessels-o…
By newseditor
Posted 06 Apr
Single-cell biclustering fo…
By newseditor
Posted 01 Apr
Modular dual-color BiAD sen…
By newseditor
Posted 31 Mar
Publications
The immunobiology of herpes…
By newseditor
Posted 17 Apr
Circulating microbiome DNA…
By newseditor
Posted 17 Apr
Spindle oscillations in com…
By newseditor
Posted 17 Apr
Oligodendroglial macroautop…
By newseditor
Posted 17 Apr
COPII with ALG2 and ESCRTs…
By newseditor
Posted 17 Apr
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar