Multiple pathways involved in taste cell generation!

Multiple pathways involved in taste cell generation!

Ever burn your tongue so badly that you were unable to taste your food for a few days? Luckily, a unique feature of taste cells is that they continually regenerate every 10 to 14 days. Now, a new study advances understanding of how stem cells on the tongue grow into the different types of mature taste cells that detect either sweet, salty, sour, bitter, or umami.

By identifying novel genes and molecular pathways involved in shaping a taste cell's function, these findings may someday allow scientists to treat taste disorders, characterize new taste qualities, or even fine-tune a person's taste perception to encourage healthier eating.

Taste cells are located in clusters called taste buds, which in turn are found in papillae, the raised bumps visible on the tongue's surface. Two different types of specialized taste cells contain the chemical receptors and intracellular molecular machinery needed to initiate the perception of taste. A third type appears to serve as a supporting cell.

In 2013, researchers identified the stem, or progenitor, cell that gives rise to these three different taste cell types. Moving forward, they were able to place these taste stem cells in a culture dish and prompt them to grow into the different mature taste cell types, thus creating a taste bud in a dish -- scientifically known as taste organoids.

In the current paper, published  in journal Scientific Reports, researchers studied taste organoids at different stages of growth to identify which genes are turned on at each stage of taste cell generation.

Using a powerful genetic technology called RNA-seq, these experiments revealed a nearly comprehensive list of all the genes, including some not previously identified, that guide the development of taste cells. The studies also revealed when during taste cell differentiation these genes influence whether a given taste cell ultimately will respond to either salty, sweet, sour, bitter or umami.

Other experiments expanded the findings to provide clues about the molecular signals that may direct the taste stem cells to go down one path or another. Using pharmacological approaches, the researchers identified the so-called signaling proteins within the immature taste cells that cause the developing cells to multiply and turn into specific cell types. These studies revealed the important roles of several signaling pathways, including ones not previously known to play a role in taste.

Authors show that transcripts of taste receptors appear only or predominantly in late-stage organoids. Prior to that, transcription factors and other signaling elements are upregulated. RNA-Seq identified a number of well-characterized signaling pathways in taste organoid cultures, such as those involving Wnt, bone morphogenetic proteins (BMPs), Notch, and Hedgehog (Hh).

By pharmacological manipulation, authors demonstrate that Wnt, BMPs, Notch, and Hh signaling pathways are necessary for taste cell proliferation, differentiation and cell fate determination. The temporal expression profiles displayed by taste organoids may also lead to the identification of currently unknown transducer elements underlying sour, salt, and other taste qualities, given the staged expression of taste receptor genes and taste transduction elements in cultured organoids.

"By better understanding how our taste cells detect and translate information about the chemical constituents of our food, we may be able to confirm how humans detect poorly-understood qualities such as fat or calcium, or even identify entirely new tastes," said study co-author.

Senior author notes that the research may have treatment implications for patients who lose their sense of taste following radiation for head and neck cancers. "Understanding how taste cells grow may help us develop novel strategies to help patients with taste disorders”.