New research indicates that the location of receptors that transmit pain signals is important in how big or small a pain signal will be and how effectively drugs can block those signals.

Blocking pain receptors in the nucleus of spinal nerve cells could control pain more effectively than interfering with the same type of receptors located on cell surfaces, the research shows. The scientists also found that when those same nerve cells encounter a painful stimulus, some of the receptors migrate from the cell surface into the nucleus.

The researchers focused on a specific type of glutamate receptor that is part of the family of receptors called G-protein-coupled receptors, which are important in signaling between neurons.

In a rat model that mimics a type of chronic, neuropathic pain, animals treated with investigational drugs to block the activity of the receptors in the nucleus responded in ways suggesting they had gotten relief from their pain.

Rats with nerve injuries displayed less spontaneous pain and less hypersensitivity to a painful stimulus when those nuclear receptors were blocked. But normal rats without nerve injuries had no changes in pain sensitivity when those receptors were blocked and the animals were exposed to a painful stimulus.

The researchers found that when these particular glutamate receptors on the nucleus of a nerve cell were activated, the response -- measured by the amount of calcium released-- was nine times larger than when the same type of receptor was activated on the cell's surface. Changes in calcium levels play a key role in signaling in neurons. Increased calcium can release important neurotransmitters, regulate specific genes and contribute to synaptic changes that are critical to pain signals.

They also found that the glutamate receptors on the nucleus responded to painful stimuli more robustly than the same types of receptors located on the cell's surface, and that when the cells encountered such a stimulus, some receptors migrated from the surface to the nucleus. The researchers also discovered that receptors located in the nucleus stopped activating pain signals when targeted with drugs.

The researchers focused mainly on nerve cells in the spinal cord, an important area for transmitting pain signals coming from all parts of the body. Future research will be aimed at determining what events cause the glutamate receptors to migrate to the nucleus and how to make drugs that more specifically block only glutamate receptors in the nucleus of the nerve cells.