Researchers have solved a medical mystery in a poorly understood disease by uncovering which cells cause tumors in patients with tuberous sclerosis complex (TSC). As described in Cell Reports, they did this by creating genetically engineered kidney organoids, or “mini-kidneys” grown from human tissue.
“The cells at the origin of tuberous sclerosis tumors have been a mystery for decades,” said senior author. “Our results can help find possible treatment targets for this challenging disease.”
TSC is a rare genetic disease that causes benign tumors in the skin, brain, kidneys, heart or lungs. TSC tumors are very diverse, arising in children or adults with a range of symptoms from mild to life-threatening and often include seizures and kidney problems. There is no cure and treatment options are limited.
“Kidney disease is the leading cause of death in patients with TSC. Around 60 to 80 percent of patients develop tumors in their kidneys, often reducing kidney function and sometimes leading to catastrophic bleeding,” said lead author. “There were no adequate lab models to study how TSC affects the kidney, so we made one ourselves.”
TSC is caused by mutations in the TSC1 or TSC2 gene. For most patients, these mutations arise spontaneously during development or early life rather than being inherited from their parents. This makes TSC a difficult disease to study. While lab researchers often use animals to study human diseases, there was no animal model that fully captured TSC’s impact on the kidneys.
TSC tumors in the kidney have puzzled experts because they are extraordinarily diverse in size, cellular makeup and gene expression, even within the same patient. What causes this diversity was unknown, and it makes treatment challenging.
To better understand the impact of TSCs on the kidneys, the team grew 3D kidney tissue in the lab from human stem cells that were genetically engineered to have a TSC1 or TSC2 mutation. Known as organoids, these miniature, simplified versions of kidneys had a genetic profile similar to TSC tumors found in patents. The researchers then took single cells from these kidney organoids and injected them into the kidneys of mice, where they grew into human TSC tumors.
Using these organoids, the researchers revealed that cells called Schwann Cell Precursors are where TSC tumors start in the kidney. They also found this single mutation affects the development of many different kinds of cells, which explains the variation in kidney tumors even within the same person.
“Not only can these ‘mini-kidneys’ help us to better understand this disease, they can also be used to test new therapies,” said the author.
https://www.cell.com/cell-reports/fulltext/S2211-1247(22)00842-7
http://sciencemission.com/site/index.php?page=news&type=view&id=publications%2Frenal-organoid-modeling&filter=22
Renal organoid modeling of tuberous sclerosis complex
- 1,161 views
- Added
Latest News
How protein synthesis in de…
By newseditor
Posted 22 Apr
Atlas of mRNA variants in d…
By newseditor
Posted 22 Apr
Mapping microbiome in metas…
By newseditor
Posted 22 Apr
Full-length mRNA packaged i…
By newseditor
Posted 22 Apr
Glucose-sensing mechanism t…
By newseditor
Posted 21 Apr
Other Top Stories
A link between childhood stress and early molars
Read more
Omega-6 fatty acid-enriched western diet induces reversible periphe…
Read more
What is Thanatosis?
Read more
Dietary fatty acids and migraine
Read more
How hunger boosts learning about food in mice
Read more
Protocols
A programmable targeted pro…
By newseditor
Posted 23 Apr
MemPrep, a new technology f…
By newseditor
Posted 08 Apr
A tangible method to assess…
By newseditor
Posted 08 Apr
Stem cell-derived vessels-o…
By newseditor
Posted 06 Apr
Single-cell biclustering fo…
By newseditor
Posted 01 Apr
Publications
Neuronal activity rapidly r…
By newseditor
Posted 22 Apr
A perspective on muscle phe…
By newseditor
Posted 22 Apr
Foxp1 suppresses cortical a…
By newseditor
Posted 22 Apr
Single-cell long-read seque…
By newseditor
Posted 22 Apr
Unlocking potential: the ro…
By newseditor
Posted 22 Apr
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar