Among the barriers to malaria eradication are asymptomatic carriers who serve as parasite reservoirs and a lack of highly sensitive diagnostic methods suitable for use in resource-limited settings. Sensitive diagnostic methods are also needed for species of malaria parasites other than Plasmodium falciparum.
The researchers developed a CRISPR-based diagnostic method to detect four species of the malarial parasite Plasmodium. The test, which uses a nucleic acid detection platform called SHERLOCK, begins with a 10-minute parasite extraction step, followed by a 60-minute species-specific detection process with readout by fluorescence or on a lateral flow strip.
The authors report that the process is optimized for field conditions and resource-limited settings, with a cost of around $0.61 per test. The reaction materials are freeze-dried into a pellet that can be rehydrated, without the need for refrigeration.
Additionally, the authors note that the P. falciparum test is capable of detecting below two parasites per microliter of blood—a threshold smaller than the World Health Organization’s recommended limit of detection for molecular testing.
According to the authors, the test enables rapid, low-cost, sensitive diagnosis of symptomatic and asymptomatic malaria carriers, particularly carriers of nonfalciparum parasite species.
https://www.pnas.org/content/early/2020/09/15/2010196117
http://sciencemission.com/site/index.php?page=news&type=view&id=publications%2Fultrasensitive-crispr&filter=22
CRISPR-based malaria test
- 1,029 views
- Added
Edited
Latest News
Bitter taste receptor activ…
By newseditor
Posted 20 Apr
Metabolic rewiring promotes…
By newseditor
Posted 18 Apr
A drug to prevent flu-induc…
By newseditor
Posted 18 Apr
New origin of deep brain waves
By newseditor
Posted 17 Apr
Starving cells hijack prote…
By newseditor
Posted 17 Apr
Other Top Stories
Epigenetics of addiction!
Read more
Extracellular vesicles involved in intracellular signaling!
Read more
Melanoma Cells Rewire to Resist Drug Treatment
Read more
New functions of hippocampus unveiled
Read more
Treating postdiet body weight rebound
Read more
Protocols
MemPrep, a new technology f…
By newseditor
Posted 08 Apr
A tangible method to assess…
By newseditor
Posted 08 Apr
Stem cell-derived vessels-o…
By newseditor
Posted 06 Apr
Single-cell biclustering fo…
By newseditor
Posted 01 Apr
Modular dual-color BiAD sen…
By newseditor
Posted 31 Mar
Publications
RNA-DNA triplexes: molecula…
By newseditor
Posted 20 Apr
Will cellular immunotherapi…
By newseditor
Posted 20 Apr
How does the microbiota con…
By newseditor
Posted 18 Apr
The integrated stress respo…
By newseditor
Posted 18 Apr
The immunobiology of herpes…
By newseditor
Posted 17 Apr
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar