Researchers have discovered two proteins that work together to determine the fate of cells in plants facing certain stresses.

Ironically, a key discovery in this finding, published recently in Nature Communications.

The researchers have been working identify a gene in the model plant Arabidopsis that could control the plants response to stressors, which can lead to the plant’s death. They had identified a protein in Arabidopsis that seemed to control whether a plant would live or die under stress conditions.

Having identified the gene was just the beginning of the story, despite being years into the journey. It would take five more years to get to this new paper.

The researchers discovered that the proteins BON-associated protein2, or BAP2, and inositol-requiring enzyme 1, or IRE1, work together when dealing with stress conditions — a matter of life and death for plant cells.

Understanding how these proteins function can help researchers breed plants that are more resilient to death conditions.

Creating plants that are more resistant to endoplasmic reticulum stress, or ER stress, has widespread implications in agriculture. If crops can be made to be more resilient in the face of drought or heat conditions, the plants stand a better chance of surviving and thriving, despite the changing climate.

Within eukaryotic cells is an organelle known as the endoplasmic reticulum, or ER. It creates proteins and folds them into shapes the cell can utilize. Like cutting up vegetables to use in a recipe, the proteins must be formed into the right shape before they can be used.

Protein making and protein folding capacity must be in balance, like a sous chef and a chef, working in tandem. If the sous chef is providing the chef with too little or too many ingredients, it throws off the balance in the kitchen.

When the ER cannot properly do its job, or the balance is thrown off, it enters a state known as ER stress. The cell will jumpstart a mechanism known as the unfolded protein response, or UPR, to decide what to do next. If the problem can be resolved, the cell will initiative life saving measures to resolve the problem. If it cannot be, the cell begins to shut down, ending its and potentially the plant’s life.

It was known that the enzyme IRE1 was responsible for directing the mechanisms that would either save the cell or kill it off. But what calls IRE1 to action?

In this study, the researchers were searching for the master regulator of these pro-death processes, known as programmed cell death.

The researchers started by looking at hundreds of accessions, or plants of the same species but specific to one locale. For example, a plant that grows in Colombia will have genetic variations to the same species of plant that grows in Spain, and the ways they each respond to stress conditions could differ.

They found extensive variation in the response to ER stress between the different accessions. Taking the accessions whose responses were the most dissimilar, they tried to identify the differences in their genomes. This is where the BAP2 gene candidate came into play.

“We found that BAP2 responds to ER stress,” said the lead author. “And the cool thing is that it is able to control and modify the activity of IRE1. But also IRE1 is able to regulate BAP2 expression.”

BAP2 and IRE1 work together, signaling to each other what the best course of action for the cell is. Having one without the other results in the death of the plant when the ER homeostasis is unbalanced.

https://www.nature.com/articles/s41467-024-50105-6

http://sciencemission.com/site/index.php?page=news&type=view&id=publications%2Fprogrammed-cell-death_4&filter=22