Liver bud-like tissue from human pluripotent stem cells

Liver bud-like tissue from human pluripotent stem cells

Human induced pluripotent stem cells (hiPSCs) have potential for personalized and regenerative medicine. While most of the methods using these cells have focused on deriving homogenous populations of specialized cells, there has been modest success in producing hiPSC-derived organotypic tissues or organoids.

Researchers present a novel approach for generating and then co-differentiating hiPSC-derived progenitors. With a genetically engineered pulse of GATA-binding protein 6 (GATA6) expression, they initiate rapid emergence of all three germ layers as a complex function of GATA6 expression levels and tissue context.

Within 2 weeks the authors obtain a complex tissue that recapitulates early developmental processes and exhibits a liver bud-like phenotype, including haematopoietic and stromal cells as well as a neuronal niche.

Collectively, this approach demonstrates derivation of complex tissues from hiPSCs using a single autologous hiPSCs as source and generates a range of stromal cells that co-develop with parenchymal cells to form tissues.

http://www.nature.com/ncomms/2016/160106/ncomms10243/full/ncomms10243.html
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