Mechanism of gene transfection using plasma irradiation

Mechanism of gene transfection using plasma irradiation

 The research team has been conducting studies on low-damage and highly-efficient gene transfection - which is an indispensable tool for the generation of iPS cells and gene therapy - using the atmospheric-pressure low-temperature plasma. While it is known that gene transfection is caused by plasma irradiation, the mechanism of the plasma gene transfection has still not been clarified.

The team found that the short-lived reactive species generated in the plasma enhances calcium influx through the transient receptor potential (TRP) channels that obviously contributes effectuating plasma actions including gene transfection.

The calcium influx has been found to be a trigger for the various cell activities. For example, the feeling of pain, heat, coldness in the neuron cell; the sensing of sweetness in the taste cell; and cellular response such as cell proliferation, cell differentiation, endocytosis and apoptosis via the signal transduction or cell signalling.

"It's a breakthrough to have found a way to control the calcium influx using plasma irradiation," says the author. "We hope the results of our research will not only contribute to the development of low-damage and highly-efficient gene transfection, but also to improving cancer therapies, blood coagulation and wound healing."