Microscopy is a basic tool in research into the biology of any organism given that the elements studied, the cells, are of microscopic and frequently nanoscopic size. Until now, existing microscopy methods to explore living brain tissue have been limited to imaging previously labelled cells only. Yet, owing to technical limitations, not all the cells in a specific region of the brain can be labelled simultaneously; this has restricted the way we see and therefore understand how brain cells, which are highly interconnected, are organised and interact with each other.
The article published in the journal Cell describes a new microscopy technique known as SUSHI designed to improve the imaging of cells in living brain tissue.
The new SUSHI (Super-resolution Shadow Imaging) technique allows the tiny space full of liquid surrounding brain cells (ECS) to be labelled in one sweep, thus obviating the need to individually label all the cells that one is intending to analyze.
SUSHI enables quantitative analysis of ECS structure and reveals dynamics on multiple scales in response to a variety of physiological stimuli. The extracellular labeling strategy greatly alleviates problems of photobleaching and phototoxicity associated with traditional imaging approaches.
Given that this "label" also remains outside the cells, a kind of negative image akin to the film used in old cameras is produced. So the negative image contains the same information about the brain cells as its corresponding positive image, but thanks to the fact that the labelling procedure is more straightforward, it is much easier to obtain this image and all the information contained in it.
According to the author "The SUSHI technique is revolutionary because it allows us to simultaneously image all the brain cells in a specific region of living brain tissue. In the past we used to come across blank spaces in the microscopy images, because we were unable to label all the cells at the same time. This fact was a big constraint for us. From now on, this technique will enable us to see all the cells in the area of study that we put under the microscope lens as well as all their interactions, and that will allow us to advance our knowledge of brain functions in a healthy organ and in a diseased one".
Brain extracellular space imaging using SUSHI
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