Investigators have developed a simple method to maintain water and water-based solutions in a liquid state at temperatures far below the usual "freezing point" for greatly extended periods of time. While they currently have accomplished this for volumes of only a few ounces, their approach - described in the journal Nature Communications - may someday enable safe, extended preservation of blood cells, tissues and organs, along with improved food preservation.
"Water and other aqueous solutions in the sorts of volumes we deal with every day normally freeze when cooled below the freezing point of 0° C or 32° F," says co-corresponding author of the report. "Our approach, which we dubbed 'deep supercooling,' is simply to cover the surface of such a liquid with a solution that does not mix with water, like mineral oil, to block the interface between water and air, which is the major site of crystallization. This surprisingly simple, practical and low-cost approach to supercooling solutions for extended periods can enable many medical and food preservation methods, as well as fundamental experiments that were not previously possible."
In most real-world environments, water and water-based solutions begin to freeze when the temperature reaches below 0° C/32° F, with ice crystals randomly forming where the liquids contact air or various impurities in the solution. Supercooling - reducing a liquid below its usual freezing point without crystallization - has been achieved for very small volumes and brief periods of time or by using high pressure equipment that is both costly and possibly damaging to tissues or other biological materials.
Reducing the temperature of any biological material - such as cold storage of perishable foods and organs for transplantation - slows down metabolic and other reactions. Supercooling extends this metabolic deceleration further without the damage caused by ice crystallization. Following upon observations the team first found that sealing the surface of a small (1 ml) water sample with a hydrocarbon-based oil - such as mineral oil, olive oil or paraffin oil - could suppress ice formation at temperatures as low as -13° C (around 9° F) for up to a week. Through a series of experiments both with more complex oils and with pure simple hydrocarbons, such as alcohols and alkanes, they succeeded in keeping 1 ml samples of water and cell suspensions supercooled at -20° C (-4° F) for 100 days and 100 ml (3.2 oz) samples for a week.
The team also demonstrated application of their deep supercooling method to the extended preservation of red blood cells. While red blood cells are usually stored at 4° C (39° F) for as long as 42 days, recent reports have suggested that cell quality at that temperature begins to decline after around 14 days, and irreversible cellular injury sets in after 28 days, challenging current blood banking practice.
The team's preliminary experiments indicated that their deep supercooling approach could safely preserve red-blood-cell suspensions of up to 100 ml at -13° C for as long as 100 days, more than doubling the current storage time.
"We currently are conducting experiments to increase the volume of red blood cell storage samples up to the more clinically relevant 300 to 500 ml range," says the author. "We also are working on applying this method to other cells and on translating it to large tissues and whole organs like the liver. Along with potential applications in medicine and food preservation, we also believe this invention could be used to study chemical reactions in the liquid state at low temperatures without the usual costly and complicated high-pressure equipment."
https://www.massgeneral.org/about/pressrelease.aspx?id=2280
https://www.nature.com/articles/s41467-018-05636-0
Can we keep liquids from freezing at very low temperatures for extended periods
- 1,459 views
- Added
Edited
Latest News
Metabolic rewiring promotes…
By newseditor
Posted 18 Apr
A drug to prevent flu-induc…
By newseditor
Posted 18 Apr
New origin of deep brain waves
By newseditor
Posted 17 Apr
Starving cells hijack prote…
By newseditor
Posted 17 Apr
Miniature battery-free epid…
By newseditor
Posted 17 Apr
Other Top Stories
Targeting treatment resistance in chronic lymphocytic leukemia
Read more
Unique tumor-related bacteria tied to young-onset colorectal cancer
Read more
How the tumor escapes therapies
Read more
How one type of lung cancer can transform into another
Read more
How sleepy Acute Myeloid Leukaemia stem cells awaken and grow?
Read more
Protocols
MemPrep, a new technology f…
By newseditor
Posted 08 Apr
A tangible method to assess…
By newseditor
Posted 08 Apr
Stem cell-derived vessels-o…
By newseditor
Posted 06 Apr
Single-cell biclustering fo…
By newseditor
Posted 01 Apr
Modular dual-color BiAD sen…
By newseditor
Posted 31 Mar
Publications
How does the microbiota con…
By newseditor
Posted 18 Apr
The integrated stress respo…
By newseditor
Posted 18 Apr
The immunobiology of herpes…
By newseditor
Posted 17 Apr
Circulating microbiome DNA…
By newseditor
Posted 17 Apr
Spindle oscillations in com…
By newseditor
Posted 17 Apr
Presentations
Hydrogels in Drug Delivery
By newseditor
Posted 12 Apr
Lipids
By newseditor
Posted 31 Dec
Cell biology of carbohydrat…
By newseditor
Posted 29 Nov
RNA interference (RNAi)
By newseditor
Posted 23 Oct
RNA structure and functions
By newseditor
Posted 19 Oct
Posters
A chemical biology/modular…
By newseditor
Posted 22 Aug
Single-molecule covalent ma…
By newseditor
Posted 04 Jul
ASCO-2020-HEALTH SERVICES R…
By newseditor
Posted 23 Mar
ASCO-2020-HEAD AND NECK CANCER
By newseditor
Posted 23 Mar
ASCO-2020-GENITOURINARY CAN…
By newseditor
Posted 23 Mar