A team of researchers has created a new technology that enhances scientists' ability to communicate with neural cells using light. The team synthesized three-dimensional fuzzy graphene on a nanowire template to create a superior material for photothermally stimulating cells. NW-templated three-dimensional (3D) fuzzy graphene (NT-3DFG) enables remote optical stimulation without need for genetic modification and uses orders of magnitude less energy than available materials, preventing cellular stress.

Graphene is abundant, cheap, and biocompatible. The lab has been working with graphene for several years, developing a technique of synthesizing the material in 3D topologies that is labeled "fuzzy" graphene. By growing two-dimensional (2D) graphene flakes out-of-plane on a silicon nanowire structure, they're able to create a 3D structure with broadband optical absorption and unparalleled photothermal efficiency.

These properties make it ideal for cellular electrophysiology modulation using light through the optocapacitive effect. The optocapacitive effect alters the cell membrane capacitance due to rapidly applied light pulses. NT-3DFG can be readily made in suspension, allowing the study of cell signaling within and between both 2D cell systems and 3D, like human cell-based organoids.

Systems like these are not only crucial to understanding how cells signal and interact with each other, but also hold great potential for the development of new, therapeutic interventions. Exploration into these opportunities, however, has been limited by the risk of cellular stress that existing optical remote-control technologies present. The use of NT-3DFG eliminates this risk by using significantly less energy, on a scale of 1-2 orders of magnitude less. Its biocompatible surface is easy to modify chemically, making it versatile for use with different cell types and environments. Using NT-3DFG, photothermal stimulation treatments could be developed for motor recruitment to induce muscle activation or could direct tissue development in an organoid system.

"The broadband absorption of these 3D nanomaterials enabled us to use light at wavelengths that can penetrate deep into the tissue to remotely excite nerve cells. This method can be used in a whole gamut of applications, from designing non-invasive therapeutics to basic scientific studies," said the author.

The team's findings are significant both for our understanding of cell interactions and the development of therapies that harness the potential of the human body's own cells. Nanostructures created using NT-3DFG may have a major impact on the future of human biology and medicine.

https://engineering.cmu.edu/news-events/news/2020/06/03-cohen-karni-pnas.html

https://www.pnas.org/content/early/2020/05/29/1919921117

http://sciencemission.com/site/index.php?page=news&type=view&id=publications%2Fremote-nongenetic&filter=22