Studying brain disorders in people and developing drugs to treat them has been slowed by the inability to investigate single living cells from adult patients. In a first-of-its-kind study published in Cell Reports researchers were able to grow adult human neurons donated from patients who had undergone surgery. From these cell cultures, they identified more than five brain cell types and the potential proteins each cell could make.
"We were surprised that we could grow these neurons at all," author said. "The oldest tissue came from a donor who was in their mid-sixties. This is even more surprising because neurons don't divide, so they need to last a lifetime. We are finally able to characterize adult aged cells from the most enigmatic organ of the body - the seat of learning and memory, as well as consciousness."
The team used tumor-free tissue from seven patients: three who underwent a temporal lobectomy, in which a portion of the temporal lobe is removed to stop epileptic seizures, and four who had glioblastoma tumors removed. (MRIs and other tests did not show any evidence of tumor or other abnormal cells in the non-tumor tissues used for the study.)
The lab received the tissue sample from each patient and immediately treated it with papain, a pineapple-derived enzyme that breaks up proteins. This procedure dissociates the neurons, and from this mixture, the team cultured the live neurons.
Single cell analyses were performed on over 300 living cells. From this the team identified five known brain cell types after three weeks in culture: oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes.
Using deep RNA sequencing, they found over 12,000 expressed genes in the cells, including hundreds of different types of RNAs specific to the different cell types. They also identified long noncoding RNAs involved in regulation of many other genes that correlated with cell type. They found that each patient's neurons had a specific gene-expression profile that was consistent between cells.
The neurons used in this study came from subjects ranging in age from their twenties to their sixties, showing that this system will permit human aging studies that have previously only been possible in rodents. "We are now testing to see how aged live neurons differ from those of a younger person so that we might investigate molecular signatures of aging," senior author said.