Scientists have gained new insights into how neurons in the brain communicate during a decision, and how the connections between neurons may help reinforce a choice.
 
The study — conducted in mice and led by neuroscientists is the first to combine structural, functional, and behavioral analyses to explore how neuron-to-neuron connections support decision-making. The findings appear in the journal Nature.

“How the brain is organized to help make decisions is a big, fundamental question, and the neural circuitry — how neurons are connected to one another — in brain areas that are important for decision-making isn’t well understood,” said a co-senior author on the paper.

In the research, mice were tasked with choosing which way to go in a maze to find a reward. The researchers found that a mouse’s decision to go left or right activated sequential groups of neurons, culminating in the suppression of neurons linked to the opposite choice.

These specific connections between groups of neurons may help sculpt decisions by shutting down neural pathways for alternative options, the author said.

The other co-seniors lab uses mice to study behavioral and functional aspects of decision-making. Typical experiments involve placing a mouse in a virtual reality maze and recording neural activity as it makes decisions. Such experiments have shown that distinct, but intermingled, sets of neurons fire when an animal chooses left versus right.

The new study focused on a region of the brain called the posterior parietal cortex — what the authors describe as an “integrative hub” that receives and processes information gathered by multiple senses to help animals make decisions.

“We were interested in understanding how neural dynamics arise in this brain area that is important for navigational decision-making,” co-senior author said. “We’re looking for rules of connectivity — simple principles that provide a foundation for the brain’s computations as it makes decisions.”

The lab recorded neural activity as mice ran a T-shaped maze in virtual reality. A cue, which happened several seconds beforehand, indicated to the mice whether a reward would be in the left or right arm of the T. The Lee lab used powerful microscopes to map the structural connections between the same neurons recorded during the maze task.  

By combining modalities, the researchers distinguished excitatory neurons — those that activate other cells — from inhibitory neurons, which suppress other cells. They found that a specific set of excitatory neurons fired when a mouse decided to turn right, and these “right-turn” neurons activated a set of inhibitory neurons that curbed activity in “left-turn” neurons. The opposite was true when a mouse decided to turn left.

“As the animal is expressing one choice, the wiring of the neuronal circuit may help stabilize that choice by suppressing other choices,” the author said. “This could be a mechanism that helps an animal maintain a decision and prevents ‘changes of mind’.”

The findings need to be confirmed in humans, although the authors expect that there is some conservation across species.

The researchers see many directions for future research. One is exploring the connections between neurons involved in decision-making in other brain regions.

We used these combined experimental techniques to find one rule of connectivity, and now we want to find others,” the author said.

https://www.nature.com/articles/s41586-024-07088-7