Microfluidic chip for specific leukocyte counting for HIV diagnosis
- 2,757 views
Researchers have developed a highly sensitive biosensor based on a differential immuno-capture technology that can detect sub-populations of white blood cells. As part of a small, disposable biochip, the microfluidic biosensor can count CD4+/CD8+ T cells quickly and accurately for AIDS diagnosis in the field.
"An important diagnostic biomarker for HIV/AIDS is the absolute count of the CD4+ and CD8+ T lymphocytes in the whole blood. The current diagnostic tool--a flow cytometer--is expensive, requires large blood volume, and a trained technician to operate," author said. "We have developed a microfluidic biosensor based on a differential immuno-capture electrical cell counting technology to enumerate specific cells in 20 minutes using 10 microliters of blood." (There are about 50 microliters in a drop of blood).
Human blood is composed of 45 percent of cells with 5 million erythrocytes as compared to only 7000 leukocytes in one microliter of blood. Specific leukocytes like CD4 T cells are of the order of 50-1000 cells per microliter. Electrical cell counting can differentiate cells based on size and membrane properties depending on the frequency of the interrogation signal. However, differentiating cells of same morphology is a challenge.
In addition to the microfluidic "capture chamber," the new chip incorporates separate ports for lysing reagents and quenching buffers that preserve the leukocytes for counting by the microfabricated electrodes. Specific leukocytes like CD4 T cells get captured as they interact with the antibodies in the capture chamber; a second counter recounts the remaining leukocytes. The difference in the respective cell counts give the concentration of the cells captured.