A new mechanism for Chlamydia to enter human cells

Chlamydia are bacteria that can infect human and animal cells. Human health is particularly affected by Chlamydia pneumoniae (Cpn) and Chlamydia trachomatis. Chlamydia pneumoniae attacks the upper and lower respiratory tract and causes bronchitis, sinusitis and chest infections. Importantly, infections with this bacterium are associated with many chronic diseases such as chronic bronchitis, asthma, atherosclerosis and Alzheimer's disease.

Chlamydia multiply only inside of human cells. To do so, they first have to bind to the host cell from the outside and then enter the cell in a second step. Each cell is surrounded by a membrane known as the 'plasma membrane'. This membrane comprises lots of individual molecules called 'phospholipids'. There are also membrane proteins, which are responsible for transporting materials between the cell interior and the outer environment as well as for intercellular communication. One component of the membrane is the phospholipid phosphatidylserine, or 'PS' for short. In healthy cells, it is located on the inner membrane leaflet, but if the cell is diseased, it is transported to the external membrane leaflet. Here, PS serves as a marker for programmed cell death (apoptosis).

A protein called LIPP was discovered in Cpn that plays a key role in the binding of the bacterium to a human cell. This protein is located on the surface of the bacterium. In earlier studies, the researchers found that a chlamydial infection with Cpn is increased substantially if LIPP is added artificially.

One of their findings was that the LIPP protein binds directly to the plasma membrane and traverses the membrane after binding. Several LIPP molecules then form a pore in the plasma membrane.

Next, however, the bound LIPP transports the PS molecule, normally located on the inner membrane leaflet, to the outside. LIPP was the first protein to be identified that transports a component of the inner membrane leaflet to the surface of the cell when added externally. Surprisingly, this does not trigger cell death. Instead, the cell remains viable. This applies both to cells confronted only with the LIPP proteins and to those also infected with Chlamydia.

The research team proposes that it is beneficial for the Chlamydium to transport the PS molecule of the host cell outward using its LIPP protein. Possibly, at the place where the PS with the LIPP protein is located on the membrane, the membrane is so deformed that the Chlamydium can enter the cell more easily. It is also possible that the PS molecules transported to the outer membrane leaflet of the human cell serve as a receptor for the bacterium.

The first author of the study, had this to say about the prospects arising from these findings: "The LIPP protein has now become a possible target to prevent a chlamydial infection. If we succeed in coming up with a drug that restricts the function of the LIPP protein, it may be possible to prevent the infection." Likewise it is now possible to examine whether other pathogens have a tool similar to Chlamydia that they use to enter human cells. "If this were the case, it could form the basis for an extensive strategy against pathogenic bacteria," emphasises the senior author.

https://www.uni-duesseldorf.de/home/startseite/news-detailansicht-inkl-gb/article/wie-chlamydien-sich-den-zugang-zu-menschlichen-zellen-verschaffen.html

https://www.nature.com/articles/s41467-019-12419-8