The yeast Saccharomyces cerevisiae is a model organism for replicative aging studies; however, conventional lifespan measurement platforms have several limitations.
Researchers in the journal Cell Reports present a microfluidics platform that facilitates simultaneous lifespan and gene expression measurements of aging yeast cells.
This multiplexed high-throughput platform offers the capability to perform independent lifespan experiments using different yeast strains or growth media. Using this platform in minimal media environments containing glucose, authors measured the full lifespan of individual yeast cells in wild-type and canonical gene deletion backgrounds.
Compared to glucose, in galactose they observed a 16.8% decrease in replicative lifespan accompanied by an ∼2-fold increase in single-cell oxidative stress levels. Authors saw that OFF and ON cells are similar in their lifespan.
This work shows that aging cells are committed to a single phenotypic state throughout their lifespan.