We've all heard the expression: "what doesn't kill you makes you stronger." Now, a new research suggests why, at a cellular level, this might be true. The team reports that brief exposures to stressors can be beneficial by prompting the cell to trigger sustained production of antioxidants, molecules that help get rid of toxic cellular buildup related to normal metabolism.

The research, which appeared in the journal Cell Metabolism, also revealed that short-term stress to cells leads to remodeling mitochondria, the powerhouses of the cell that deteriorate with age, so they generate fewer toxic byproducts. The findings could lead to new approaches to counter the cellular effects of aging, possibly even extending lifespan.

"The novelty of this study is that we've generated a model in which we can turn off antioxidant production in mitochondria but in a reversible way," says the senior author of the paper. "So we were able to induce this stress for specific time windows and see how cells responded."

In the process of converting food into chemical energy, mitochondria produce a chemical called superoxide, which has a critical role in cells but is toxic if it builds up. For this reason, mitochondria also produce an enzyme--superoxide dismutase, or SOD--to convert superoxide to a less toxic form.

Authors wanted to know how short-term cellular stress caused by mitochondrial superoxide very early in development might affect health later in life. So the team developed an approach to turn off the SOD enzyme for short periods of time in order to study how cells and animals responded to the cellular stress of toxic buildup.

In a group of genetically identical mice in utero, half with a molecular "off" switch for SOD experienced brief stress when the enzyme was deactivated. After the mice were born and continued to grow to adulthood, the two groups looked very similar. But liver samples taken when they were four weeks old told a strikingly different story: the mice whose SOD enzyme had been turned off briefly to trigger stress in mitochondria had--surprisingly--higher levels of antioxidants, more mitochondria and less superoxide buildup than the mice who had not experienced stress. Additionally, cells grown in dishes, half which contained the SOD switch, showed the same results: those that experienced brief periods of stress turned out to be stress resistant and healthier from a cellular perspective.

When the team analyzed which genes were being activated in both the lab dishes and the liver samples of all the mice, they found unexpected molecular pathways at work in the SOD group that were reprogramming mitochondria to produce fewer toxic molecules while simultaneously increaseing the cells' antioxidant capacity. Gene expression analysis implicated non-canonical activation of the Nrf2 antioxidant and PPARγ/PGC-1α mitochondrial signaling pathways in this response. 

The work suggests that short-term mitochondrial stress may lead to long-term adaptations (a concept called "mitohormesis") that could keep cells healthy longer, staving off aging and disease. Authors next plans to study whether the mechanism elucidated here can delay the effects of aging in mammals.

The senior author adds, "We are excited to test if the unique mitohormesis signaling pathways we will elucidate in this new mouse model can be targeted to prevent common age-related disease like cancer, Alzheimer's and heart disease."

https://www.salk.edu/news-release/cells-agree-what-doesnt-kill-you-makes-you-stronger/

https://www.cell.com/cell-metabolism/fulltext/S1550-4131(18)30454-6