CRISPR screening of cell–cell interactions by SPEAC-seq
SPEAC-seq uses CRISPR screening, cell coculture in droplets and microfluidic sorting to enable forward genetic screens of regulators of cell–cell communication.
The procedure provides a detailed experimental workflow and discusses technical aspects. SPEAC-seq enables the defined pairing of interaction partners, is adaptable to different CRISPR screening modalities, is scalable for unbiased genome-wide discovery of functional regulators and can be adapted with few modifications to study a variety of cell types
