Enhancing human brain organoid physiology
The initial state of pluripotent stem cells influences brain organoid formation and inherent differences may necessitate design of tailored protocols to enhance reproducibility.
Brain organoid architecture is influenced by various factors, including physical forces, extracellular matrix (ECM), and morphogen signaling, and approximating tissue-like architecture may increase cell identity and functionality.
Early and nuanced changes in brain organoid protocols can have major outcomes at the level of morphology, growth, and increased cellular diversity, such as more abundant outer radial glia cells.
Guided by in vivo signaling, the presence of under-represented cell populations can be increased and organoid xenotransplantation can promote further maturation, vascularization, and integration within host brain networks.
https://www.cell.com/trends/cell-biology/fulltext/S0962-8924(24)00254-X
