Transcription-coupled repair of DNA–protein crosslinks

New techniques for studying DNA– protein crosslink (DPC) repair enable the identification of crosslinked proteins and mapping of DPCs to genomic loci. 

Recent studies have uncovered the existence of a transcription-coupled (TC)- DPC repair pathway.

TC-DPC repair is initiated upon RNA polymerase II stalling and recruitment of the Cockayne syndrome (CS) proteins CSB and CSA which have well-characterized roles in transcription-coupled nucleotide excision repair (TC-NER) of UV-induced DNA lesions.

TC-DPC repair is thought to occur through CSA-dependent DPC polyubiquitylation and proteasomal degradation, although the precise molecular events downstream of CSA recruitment remain unclear.

TC-DPC repair is independent of downstream TC-NER machinery, possibly explaining some of the disparity between CS and diseases characterized by loss of NER.

https://www.cell.com/trends/cell-biology/fulltext/S0962-8924(24)00234-4

https://sciencemission.com/Transcription-coupled-repair-of-DNA%E2%80%93protein-crosslinks