An organ-chip model of sporadic ALS

Motor neurons (MNs) degenerate in amyotrophic lateral sclerosis (ALS).   Early stages of disease could be elucidated by generating MNs from patient-specific induced pluripotent stem cells (iPSCs).

IN this study, the researchers differentiate iPSCs from young onset, sporadic ALS patients into spinal cord motor neurons and co-culture them with brain microvascular endothelial-like cells in a microfluidic organ-chip system.

Transcriptomics, proteomics, and single nuclei RNA sequencing identify distinct motor neuron subpopulations with ALS specific dysregulation in neurofilaments, glutamatergic, and synaptic signaling.

https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(25)00222-X

https://sciencemission.com/An-organ-chip-model-of-sporadic-ALS