snoRNA-facilitated protein secretion
Dynamic, reversible modifications of DNA and RNA regulate how genes are expressed and transcribed, which can influence cellular processes, disease development, and overall organismal health. Small nucleolar RNAs (snoRNAs) are a common but overlooked group of guide RNA molecules that steer chemical modifications to cellular ribosomal RNA (rRNA) targets, like an usher showing someone to their seat in a theater.
Researchers recently developed a new approach for identifying new cellular RNA targets of snoRNAs. They uncovered thousands of previously unknown targets for snoRNAs in human cells and mouse brain tissues, including many that serve functions other than guiding rRNA modifications. Some of the newly discovered interactions with messenger RNA (mRNA) facilitate protein secretion, an important cellular process that could be harnessed for potential therapeutics and biotechnology applications.
“Once you see so many targets for these snoRNAs, you realize there’s a lot more to be understood,” said a co-senior author of the paper. “We already see that they play a role in protein secretion, which has major implications for physiology, and it suggests a path forward to study hundreds of other snoRNAs.”
The paper was published in the journal Cell.
There are more than 1,000 known genes for encoding snoRNAs in the human genome, but scientists have only pinpointed the RNA targets for about 300 of them. These targets mostly involve guiding modifications for ribosomal RNA and small nuclear RNA involved in mRNA splicing. In the decades since snoRNAs were first discovered, researchers largely left the remaining 700 alone, assuming they performed similar functions. However, unlike other guide RNA molecules such as microRNAs that are all the same length, snoRNAs vary greatly in their length from 50-250 residues, suggesting that they can do many different things.
Most of the newly discovered snoRNA targets do not overlap with the known RNA modification sites, suggesting that snoRNAs may have a much broader function in cells. One unexpected discovery was that a snoRNA called SNORA73 interacts with mRNAs that encode secreted proteins and cell membrane proteins. Protein secretion is a fundamental biological process by which proteins are transported from a cell into the extracellular space, which is crucial for various functions, including communication between cells, immune responses, and digestion. The researchers saw that SNORA73 acts as a “molecular glue” between the mRNA and the protein synthesis machinery that helps facilitate this process.
Further analysis of how SNORA73 binds with mRNA suggested that synthetic snoRNA sequences can be engineered to affect protein secretion. The researchers tested this hypothesis by tweaking a green fluorescent protein (GFP) reporter to interact with SNORA73. GFPs are often introduced in cells to make them glow under certain conditions so scientists can see the effects of experiments. When the researchers expressed SNORA73 genes with the engineered GFP that can be secreted from cells, it increased protein secretion by 30 to 50% over controls.
These experiments showed that they could make use of the snoRNA machinery to manipulate the secretion of a given protein, which could be useful for developing therapeutics. For example, if a human disease involves a deficiency of secreted proteins, then bioengineers could hijack the system to deliver artificial snoRNAs to increase secretion of that protein.
While the technology for synthesizing and delivering snoRNAs to the right locations isn’t quite ready yet, both He and Pan feel confident those challenges can be solved since it builds upon previous advances in technology using other forms of RNA. They also believe that since snoRNAs are specific to cell types, they could have much more diverse functions—and therapeutic possibilities—elsewhere.
“Think about neuronal cells, stem cells, or cancer cells. There are just so many cell types one can study. So, I think the field is wide open,” the author said.
