AI tool for microglial phagocytosis analysis
Microglia are brain immune cells and microglial phagocytosis play crucial role in brain health and disease. Dysregulation of phagocytosis is implicated in various neurological disorders.
The researchers. introduce AIstain, a U-Net-based deep learning approach for label-free microglial detection in live cell image cytometry.
The method outperforms fluorescence staining and common segmentation tools, enabling accurate, high-throughput phagocytosis analysis while avoiding cytotoxic dyes.
The model can be extended to other cell types, including leukemia and breast cancer cells.
https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(25)00243-7
