Mitochondrial protein import
Mitochondrial function depends on correct import and sorting of cytosolically synthesized proteins into their appropriate subcompartments, such as the mitochondrial matrix.
The import of most of these proteins is facilitated by the translocase of the inner membrane 23 (TIM23) complex, a mitochondrial translocase that allows the sorting of precursor proteins across the inner mitochondrial membrane (IMM).
The prevailing model of TIM23-mediated protein transport, informed by years of biochemical and genetic research, is coming under scrutiny due to new structural data.
Recent cryo-electron microscopy (EM) structures of the TIM23 complex, supporting by cross-linking data, indicate a paradigm shift in our understanding of mechanism of protein import via the TIM23 complex.
Contrasting evidence requires us to reevaluate our knowledge of the field and view previous data in the context of new knowledge.
https://www.cell.com/trends/biochemical-sciences/fulltext/S0968-0004(25)00050-7
