Protein conformations with limited proteolysis coupled to mass spectrometry
Significant improvements in limited proteolysis coupled to mass spectrometry (LiP-MS) protocols, including advanced digestion and data acquisition methods, have expanded its capabilities for structural proteomics.
LiP-MS has been used in diverse experimental models, including mammalian cells, microbial, fungal, and plant systems, and also biofluids, offering new insights into protein function and interactions.
LiP-MS can be used to investigate disease mechanisms, offering insights into protein dynamics in conditions such as cancer and neurodegenerative diseases.
Recent studies leverage LiP-MS for drug–target interaction profiling to aid the discovery and development of novel therapeutics.
LiP-MS is expected to advance structural proteomics and (bio)medical research, thereby aiding biomarker discovery and therapeutic target identification.
https://www.cell.com/trends/biochemical-sciences/fulltext/S0968-0004(24)00257-3
