Selecting Escherichia coli strains for growth-coupled metabolic engineering

‘Designer’ metabolism could enhance carbon capture, bioremediation, and bioproductions. 

Transferring synthetic metabolism into living cells is challenging.

Coupling cell growth to the pathway of interest in metabolically rewired selection strains advances the implementation of synthetic metabolism.

Growth rates and biomass yields can be used to approximate pathway turnover or compare pathway efficiencies.

A selection strain must be thoroughly validated before use.

Selection strains covering E. coli’s central, amino acid, and energy metabolism are readily available in the community.

https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(25)00252-5

https://sciencemission.com/Escherichia-coli-selection-strains