Imaging methods for whole-brain mammalian connectomics

Imaging connectomes to reconstruct cellular morphology and synapses is important to understand brain function and pathophysiology. 

Reconstruction of whole-brain connectomes require technological advances.

The researchers quantitatively compare imaging technologies like electron microscopy (EM) techniques and expansion light-sheet fluorescence microscopy (ExLSFM) methods for their potential to enable whole-brain mammalian connectomics.

They consider techniques that have sufficient resolution to identify all synapses and sufficient speed to be relevant for whole mammalian brains.

This analysis would serve as a resource for those considering how to organize efforts toward imaging whole-brain mammalian connectomes.

https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(25)00024-4

https://sciencemission.com/imaging-methods-for-whole-brain-mammalian-connectomics