Fluorescence anisotropy for endogenous gene expression detection

High costs and technological barriers are associated with high-throughput gene expression detection assays and  gene reporter assays measure only promoter activity.

Therefore researchers developed describe a high-throughput, amplification-free, and accessible method—cellular fluorescence anisotropy of RNA (CFAR) for endogenous gene expression detection.

In a high-throughput chemical screen for oncogene MYC expression modulators, CFAR identified hits more robustly than the traditional gene reporter assay.

CFAR chemical screening reveals FDA-approved drugs targeting oncogene MYC expression in colorectal cancer cells.

https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(26)00317-6

https://sciencemission.com/gene-expression-perturbations-via-fluorescence-anisotropy