Human cerebellar organoids with functional Purkinje cells
A model system to evaluate the human cerebellum’s cellular diversity and functional feature is lacking.
The researchers developed (human cerebellar organoids [hCerOs]), a human organoid model replicating the fetal cerebellum’s cellular diversity, including the generation of human-specific rhombic lip progenitors recently linked to medulloblastoma initiation.
hCerOs can transiently form laminar organized layering and develop functional neurons, including Purkinje cells, providing an all human, physiologically relevant model system for cerebellar research.
Long-term culture of hCerOs allows healthy survival and maturation of Purkinje cells that display molecular and electrophysiological hallmarks of their in vivo counterparts.
https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(23)00430-7
