How to track newly synthesised proteins in plants
Newly synthesised proteins provide a real-time readout of protein production beyond steady-state protein abundance.
Newly synthesised protein analysis reveals dynamic proteome regulation during environmental and developmental responses.
Ribosome profiling, isotope labelling, and bioorthogonal noncanonical amino acid tagging provide complementary strategies to assess protein synthesis.
Bioorthogonal noncanonical amino acid tagging, when combined with click chemistry, enables visualisation, enrichment, and identification of newly synthesised proteins.
Newly synthesised protein analysis has strong potential for studying plant stress, development, circadian regulation, and plant–microbe interactions.
Clickable noncanonical amino acids and biorthogonal reactions enable flexible labelling strategies across experimental systems.
https://www.cell.com/trends/plant-science/fulltext/S1360-1385(26)00303-1
