Single-cell in-vivo ribosome profiling!

Low overall protein-synthesis rate drives the stemness of somatic stem cells but the aging effect on stem cells is nor clear yet. 

The researchers in this study present an in vivo single-cell ribosome profiling strategy to monitor tissue-wide translational landscapes of the epidermis during aging.

In vivo single-cell ribosome profiling reveals cell-type-specific translational landscapes across the epidermis.

identify a pronounced translational reprogramming of AP-1 subunits specifically in aged epidermal stem cells. They also uncover selective reprogramming of AP-1 subunit translation in aged stem cells, thus linking translational deregulation to stem cell aging.

https://www.cell.com/molecular-cell/fulltext/S1097-2765(26)00271-6

https://sciencemission.com/In-vivo-single-cell-ribosome-profiling