Monitoring dynamic single-cell events using noninvasive methods
Technological innovations enable real time monitoring and repeated measurements of live-cell readouts for kinase activities, metabolism, Ca2+ signaling, protein clustering, and transcriptional regulation.
Nanoprobes allow direct physical sampling of intracellular contents in a minimally invasive manner. These methods provide unique biochemical information with the potential for preserving cell viability.
Monitoring the same cell over hours or days without disrupting its physiology remains challenging for many dynamic molecular processes. The standards for noninvasiveness should be high for robust and accurate analyses.
Transparent discussions of best practices as well as pitfalls will aid broad applications of available techniques.
https://www.cell.com/trends/cell-biology/fulltext/S0962-8924(26)00055-3
